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A Microfluidic-based Hydrodynamic Trap for Single Particles

A Microfluidic-based Hydrodynamic Trap for Single Particles

Published on: January 21, 2011

Controlled droplet discretization and manipulation using membrane displacement traps.

S Padmanabhan¹, T Misteli, D L DeVoe

¹Department of Chemical and Biomolecular Engineering, University of Maryland, College Park, Maryland, USA.

|October 10, 2017

Summary

This study introduces a new platform for precise aqueous droplet manipulation using membrane displacement traps. The technology enables accurate sample discretization and droplet pairing for biological applications.

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Area of Science:

Microfluidics
Biotechnology
Materials Science

Background:

Complex manipulation of aqueous droplets is essential for various applications, including biological assays and chemical synthesis.
Existing microfluidic platforms often face limitations in precise droplet control and multi-functional operations.

Purpose of the Study:

To present an innovative microfluidic platform for advanced aqueous droplet discretization and manipulation.
To demonstrate the platform's capabilities in handling individual droplets for applications like single-cell analysis.

Main Methods:

Utilizing multi-layer polydimethylsiloxane (PDMS) devices with membrane displacement trap arrays.
Characterizing performance based on capillary number, membrane actuation pressure, trap geometry, and droplet volume.
Employing the platform for sample digitization, droplet metering, and droplet merging.

Main Results:

Established operational domains for droplet discretization, release, metering, capture, and merging.
Demonstrated reliable manipulation of nanoliter-scale aqueous droplets.
Successfully discretized a dilute bacteria sample, isolated single bacteria into droplets, and paired bacteria.

Conclusions:

The developed platform offers a novel and effective approach to sample digitization and droplet manipulation.
The system's versatility and precision are suitable for advanced biological and chemical applications.
This technology facilitates precise control over individual droplets for downstream analyses.