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Updated: Feb 21, 2026

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Difference FTIR Studies of Substrate Distribution in Triosephosphate Isomerase

Hua Deng1, Jayson Vedad2, Ruel Z B Desamero2

  • 1Department of Biochemistry, Albert Einstein College of Medicine , Bronx, New York 10461, United States.

The Journal of Physical Chemistry. B
|October 10, 2017
PubMed
Summary

Triosephosphate isomerase (TIM) enzyme kinetics reveal dihydroxyacetone phosphate (DHAP) is dominant at equilibrium. Isotope-edited FTIR shows varying DHAP substates in the Michaelis complex, suggesting potential parallel reaction pathways.

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