Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparison of Efficacy Between Lymphoplasmapheresis and Plasma Exchange in Myasthenic Crisis.

Muscle & nerve·2025
Same author

A novel Wnt/β-catenin signaling gene signature for progression and metastasis of gastric cancer.

Oncology research·2025
Same author

Heterocyclic Conjugated Polydentate Ligands Facilitate Interfacial Charge Transfer of Air-Processed Carbon-Based All Inorganic Perovskite Solar Cells with an Efficiency Over 15.

Small (Weinheim an der Bergstrasse, Germany)·2025
Same author

Biological Effects of Dietary Restriction on Alzheimer's Disease: Experimental and Clinical Investigations.

CNS neuroscience & therapeutics·2025
Same author

Pre- or post-chemotherapy: effect on PSMA uptake.

EJNMMI research·2025
Same author

Co-precipitation enrichment of Pb<sup>2+</sup> in water by ball-milling-enhanced diatomite: synergistic effects of crystal structure and surface activity.

Environmental geochemistry and health·2025

Related Experiment Video

Updated: Feb 21, 2026

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets
09:24

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets

Published on: October 3, 2014

15.2K

Magnetically Controlled Growth-Factor-Immobilized Multilayer Cell Sheets for Complex Tissue Regeneration.

Wenjie Zhang1,2, Guangzheng Yang1,2, Xiansong Wang3

  • 1Department of Prosthodontics, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, 639 Zhizaoju Road, Shanghai, 200011, China.

Advanced Materials (Deerfield Beach, Fla.)
|October 10, 2017
PubMed
Summary

This study introduces novel magnetic nanoparticles for "growth-factor-immobilized cell sheets" to improve stem cell regeneration. These engineered cell sheets show promise for bone and cartilage tissue repair in regenerative medicine.

Keywords:
graphene oxidegrowth factorsmagnetic nanoparticlesstem cell sheetstissue regeneration

More Related Videos

Construction of a Multilayered Mesenchymal Stem Cell Sheet with a 3D Dynamic Culture System
07:44

Construction of a Multilayered Mesenchymal Stem Cell Sheet with a 3D Dynamic Culture System

Published on: October 20, 2018

7.9K
Engineering a Bilayered Hydrogel to Control ASC Differentiation
07:48

Engineering a Bilayered Hydrogel to Control ASC Differentiation

Published on: May 25, 2012

14.5K

Related Experiment Videos

Last Updated: Feb 21, 2026

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets
09:24

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets

Published on: October 3, 2014

15.2K
Construction of a Multilayered Mesenchymal Stem Cell Sheet with a 3D Dynamic Culture System
07:44

Construction of a Multilayered Mesenchymal Stem Cell Sheet with a 3D Dynamic Culture System

Published on: October 20, 2018

7.9K
Engineering a Bilayered Hydrogel to Control ASC Differentiation
07:48

Engineering a Bilayered Hydrogel to Control ASC Differentiation

Published on: May 25, 2012

14.5K

Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Tissue Engineering

Background:

  • Scaffold-free cell-sheet technology is crucial for stem cell-based regeneration.
  • Growth factors are vital for directing stem cell differentiation and enhancing tissue repair.
  • A key challenge is effectively incorporating growth factors into cell sheets to boost regeneration.

Purpose of the Study:

  • To develop a novel method for creating magnetically controlled, growth-factor-immobilized cell sheets.
  • To engineer magnetic nanoparticles for labeling stem cells and delivering growth factors.
  • To enhance stem cell-based tissue regeneration using these novel cell sheets.

Main Methods:

  • Developed nanoscale graphene oxide-coated Fe3O4 magnetic nanoparticles (nGO@Fe3O4).
  • Labeled dental-pulp stem cells (DPSCs) with nGO@Fe3O4 without affecting viability.
  • Utilized magnetic force to pattern multilayered MNP-labeled cell sheets.
  • Immobilized bone morphogenetic protein-2 (BMP2) onto nGO@Fe3O4 for growth factor delivery.
  • Constructed an integrated osteochondral complex using DPSCs with BMP2 and TGFβ3.

Main Results:

  • nGO@Fe3O4 nanoparticles were successfully internalized by DPSCs.
  • Magnetic force enabled controlled organization of MNP-labeled cells into patterned cell sheets.
  • Graphene oxide coating facilitated efficient binding and delivery of BMP2.
  • BMP2-loaded cell sheets significantly enhanced bone formation.
  • Constructed functional osteochondral tissue using the engineered cell sheets.

Conclusions:

  • The developed nGO@Fe3O4 magnetic nanoparticles offer an effective platform for growth factor delivery in cell sheets.
  • This magnetically controlled cell-sheet approach shows significant potential for advancing regenerative medicine, particularly in bone and cartilage repair.
  • The technique provides a promising strategy for optimizing tissue engineering and regenerative therapies.