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Updated: Aug 11, 2026

Cryo-EM and Single-Particle Analysis with Scipion
Published on: May 29, 2021
Achieving better-than-3-Å resolution by single-particle cryo-EM at 200 keV
Mark A Herzik1, Mengyu Wu1, Gabriel C Lander1
1Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California, USA.
Abstract:
Nearly all single-particle cryo-EM structures resolved to better than 4-Å resolution have been determined using 300-keV transmission electron microscopes (TEMs). We demonstrate that it is possible to obtain reconstructions of macromolecular complexes of different sizes to better than 3-Å resolution using a 200-keV TEM. These structures are of sufficient quality to unambiguously assign amino acid rotameric conformations and identify ordered water molecules.
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