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Related Experiment Videos

A new method for studying cryptococcosis in a murine model using 99mTc-Cryptococcus gattii.

Marliete Carvalho Costa1, Lays Murta Mata2, Noelly de Queiroz Ribeiro1

  • 1Laboratório de Micologia, Departamento de Microbiologia, ICB - UFMG.

Medical Mycology
|October 10, 2017
PubMed
Summary

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Assessment of Translocation of Lacticaseibacillus rhamnosus CGMCC 1.3724 Radiolabeled with Technetium-99 m Isotope in an Experimental Model of Immunosuppression.

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A new method using technetium-99m (99mTc)-labeled Cryptococcus gattii allows for earlier detection of fungal dissemination in animal models. This radiolabeling technique improves quantification of fungal load, aiding in cryptococcosis research.

Area of Science:

  • Mycology
  • Infectious Diseases
  • Medical Imaging

Background:

  • Cryptococcus gattii causes cryptococcosis, a serious systemic mycosis with high mortality, particularly when it disseminates to the central nervous system.
  • Animal models are crucial for studying C. gattii pathogenesis and evaluating new treatments.
  • Current methods for quantifying fungal dissemination, like tissue culturing, lack sensitivity for early-stage detection.

Purpose of the Study:

  • To develop and validate a novel, more sensitive method for quantifying Cryptococcus gattii dissemination in vivo.
  • To assess the utility of radiolabeling C. gattii for early detection of fungal spread.

Main Methods:

  • Efficient radiolabeling of a C. gattii strain with technetium-99m (99mTc) without compromising cell viability.

Related Experiment Videos

  • Infection of mice via intratracheal and intravenous routes using 99mTc-labeled C. gattii for biodistribution studies.
  • Comparison of detection times between the radiolabeling method and traditional colony-forming unit (CFU) counts.

    • Main Results:

    • Technetium-99m-labeled C. gattii (99mTc-C. gattii) successfully detected yeast in the mouse brain as early as 6 hours post-inoculation.
    • Traditional CFU methods only detected C. gattii in the brain 24 hours post-infection.
    • The radiolabeling method demonstrated superior sensitivity and earlier detection of fungal dissemination.

    Conclusions:

    • Radiolabeling C. gattii with 99mTc provides a highly sensitive and rapid method for quantifying fungal dissemination in animal models.
    • This technique offers an alternative approach for future research on antifungal efficacy, fungal virulence, and host-pathogen interactions in cryptococcosis.