Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Immunogold Electron Microscopy

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A Fungal Bioluminescent Pathway (FBP)-Based Yeast Biosensor for Caffeic Acid Quantification in Food and Beverages.

ACS sensors·2026

Same author

A coelenterazine-dependent luciferase from the deep-sea coral Anthoptilum murrayi.

Biochemical and biophysical research communications·2026

Same author

Caffeylpyruvate hydrolase from the bioluminescent fungus Neonothopanus gardneri is the key recycling enzyme in the fungal bioluminescence pathway.

The FEBS journal·2026

Same author

Fungal oxyluciferin is recycled by caffeylpyruvate hydrolases.

The FEBS journal·2026

Same author

Velamins: green-light-emitting calcium-regulated photoproteins isolated from the ctenophore Velamen parallelum.

The FEBS journal·2025

Same author

Diversity, Distribution, and Evolution of Bioluminescent Fungi.

Journal of fungi (Basel, Switzerland)·2025

Search research articles

Related Experiment Video

Updated: Feb 21, 2026

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Published on: March 7, 2018

Immunoassay for Human IgG Using Antibody-functionalized Silver Nanoparticles.

Daniela M Batistela¹, Cassius V Stevani¹, Renato S Freire¹

¹Department of Fundamental Chemistry, Institute of Chemistry, University of São Paulo (USP).

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry

|October 11, 2017

Summary

A novel colorimetric immunoassay effectively quantifies human immunoglobulin G (hIgG) using silver nanoparticles. This method offers a simple, sensitive detection of hIgG in biological samples.

Keywords:

Colorimetric immunoassay aggregation inhibition antibody–antigen interactions colloidal system silver nanoparticles

More Related Videos

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform

Published on: November 10, 2016

Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α

Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α

Published on: June 14, 2018

Related Experiment Videos

Last Updated: Feb 21, 2026

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Published on: March 7, 2018

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform

Published on: November 10, 2016

Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α

Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α

Published on: June 14, 2018

Area of Science:

Biochemistry
Nanotechnology
Analytical Chemistry

Background:

Accurate quantification of human immunoglobulin G (hIgG) is crucial for diagnosing various diseases.
Existing immunoassay methods can be complex and time-consuming.
Development of simple, rapid, and sensitive assays is needed.

Purpose of the Study:

To develop a simple colorimetric immunoassay for quantifying human immunoglobulin G (hIgG).
To utilize the aggregation inhibition of antibody-functionalized silver nanoparticles (AgNP) for hIgG detection.

Main Methods:

Silver nanoparticles (AgNP) were functionalized with anti-hIgG antibodies.
The aggregation of these functionalized AgNPs was measured using the ratio of attenuance values at 400 and 530 nm (A400/A530).
The assay's response to varying concentrations of hIgG was analyzed.

Main Results:

A linear correlation was observed between the A400/A530 ratio and hIgG concentration within the range of 25-200 ng mL⁻¹.
The developed immunoassay demonstrated a low detection limit for hIgG, estimated at 11 ng mL⁻¹.
The assay is characterized by its simplicity and colorimetric readout.

Conclusions:

The described colorimetric immunoassay provides a simple and sensitive method for hIgG quantification.
The use of anti-hIgG functionalized AgNPs and aggregation inhibition offers a robust detection strategy.
This assay has potential applications in clinical diagnostics and research settings.