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Towards a compact and precise sample holder for macromolecular crystallography.

Gergely Papp1, Christopher Rossi1, Robert Janocha1

  • 1European Molecular Biology Laboratory, Grenoble Outstation, 71 Avenue des Martyrs, CS 90181, 38042 Grenoble, France.

Acta Crystallographica. Section D, Structural Biology
|October 11, 2017
PubMed
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New sample holders and pucks improve crystal storage density and positioning precision for macromolecular crystallography. These advancements support high-throughput beamlines and automated systems, enhancing data collection efficiency.

Area of Science:

  • Structural Biology
  • Crystallography
  • Biophysics

Background:

  • Current sample holders in macromolecular crystallography have limitations in storage density and crystal-positioning precision.
  • These limitations hinder the efficiency of high-throughput beamlines and the integration of automated harvesting systems.
  • Precise crystal positioning is crucial for automated alignment at beamlines, especially with rapid data collection times.

Purpose of the Study:

  • To develop a compact and precise sample holder system for macromolecular crystallography.
  • To enhance storage density and crystal-positioning accuracy for automated handling and beamline alignment.
  • To create a standardized and interoperable system for sample handling across different facilities.

Main Methods:

  • Design and prototype manufacturing of new sample holder designs (NewPin and miniSPINE).
Keywords:
NewPinSPINEplusSmartMagnethigh densityhigh precisionminiSPINEsample holder

Related Experiment Videos

  • Testing with robotic sample changers and validation on synchrotron beamlines.
  • Development of corresponding pucks with human-readable codes, machine-readable codes, and RFID tags for identification.

    • Main Results:

    • Two new sample holder designs, NewPin and miniSPINE, were developed, fitting 36 holders per puck (252 samples per dewar).
    • NewPin offers high precision (up to 10 µm) but requires a specific goniometer socket; miniSPINE is compatible with the SPINE standard.
    • An upgraded SPINE holder (SPINEplus) was proposed for a homogenous and interoperable system; pilot beamlines are being equipped for evaluation.

    Conclusions:

    • The developed sample holder system addresses limitations in storage density and precision for macromolecular crystallography.
    • miniSPINE offers a practical solution for initial integration due to its compatibility with existing standards.
    • The new system facilitates efficient high-throughput data collection and integration with automated sample handling technologies.