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Herpes simplex type 1 defective interfering particles do not affect the antiviral activity of acyclovir, foscarnet

J G Harmenberg1, L T Svensson

  • 1Department of Virology, National Bacteriological Laboratory, Stockholm, Sweden.

Acta Virologica
|March 1, 1988
PubMed

Insights

This study analyzed herpes simplex virus type 1 defective interfering particles (DI-particles) over multiple passages. No correlation was found between DI-particle concentration and the effectiveness of common antiviral drugs.

Area of Science:

  • Virology
  • Microbiology

Background:

  • Defective interfering particles (DI-particles) are variants of viruses that can affect viral replication.
  • Understanding DI-particle dynamics is crucial for antiviral therapy development.

Purpose of the Study:

  • To investigate the concentration of herpes simplex virus type 1 (HSV-1) DI-particles during serial undiluted passages.
  • To determine if DI-particle concentration correlates with the efficacy of antiviral agents.

Main Methods:

  • Herpes simplex virus type 1 (HSV-1) was serially passaged fifteen times in undiluted form using green monkey kidney cells.
  • DI-particle concentration was quantified using electron microscopy and plaque titration.
  • The activity of antiviral substances (acyclovir, foscarnet, adenine arabinoside) was assessed.

Main Results:

  • Serial undiluted passages of HSV-1 did not lead to a discernible change in DI-particle concentration.
  • No relationship was observed between the quantified DI-particle levels and the inhibitory activity of acyclovir, foscarnet, or adenine arabinoside.

Conclusions:

  • DI-particle concentration in HSV-1 does not appear to influence the in vitro activity of common antiviral drugs.
  • Further research may be needed to elucidate the role of DI-particles in viral pathogenesis and treatment response.

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