Dopamine and apomorphine do not modulate the uptake of [3H]D-aspartate in the rat striatum in-vitro
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I O'Connor1, R F Cowburn, J A Hardy
1Biochemistry Department, St. Mary's Hospital Medical School, London, UK.
The Journal of Pharmacy and Pharmacology
|April 1, 1988
Abstract:
Sodium-dependent [3H]D-aspartate uptake was measured in rat striatal homogenates. The uptake was inhibited by both L- and D-glutamate, with IC50 values of 5.6 and 224 microM, respectively. Dopamine (10(-7)-10(-4) M), apomorphine (10(-7) M), sulpiride (10(-6) M) or a combination of dopamine and sulpiride were found not to affect the observed uptake of [3H]D-aspartate. Thus, the in-vitro dopaminergic modulation of high affinity glutamate uptake reported in the literature is not found when [3H]D-aspartate is used instead of [3H]L-glutamate.