Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Prostaglandins and chronic diarrhoea: methodological problems.

K Bukhave, J Rask-Madsen

    Scandinavian Journal of Gastroenterology. Supplement
    |January 1, 1979
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Non-haem iron availability from pork meat: Impact of heat treatments and meat protein dose.

    Meat science·2011
    Same author

    Systematic review: coxibs, non-steroidal anti-inflammatory drugs or no cyclooxygenase inhibitors in gastroenterological high-risk patients?

    Alimentary pharmacology & therapeutics·2006
    Same author

    Expression of Toll-like receptor 9 and response to bacterial CpG oligodeoxynucleotides in human intestinal epithelium.

    Clinical and experimental immunology·2005
    Same author

    Activation of nuclear factor kappaB in colonic mucosa from patients with collagenous and ulcerative colitis.

    Gut·2005
    Same author

    Soluble mediators and the interaction of drugs in IBD.

    Drugs of today (Barcelona, Spain : 1998)·2004
    Same author

    Fish oil feeding is associated with an increased accumulation of dietary lipids in enterocytes: results from an in vivo study in rats.

    Scandinavian journal of gastroenterology·2003
    Same journal

    Abstract from the 41st Nordic Gastroenterology Congress, 8-11 June 2010, Copenhagen, Denmark.

    Scandinavian journal of gastroenterology. Supplement·2010
    Same journal

    Abstracts from the XL Nordic Meeting of Gastroenterology, June 8-11, 2009, Stavanger, Norway.

    Scandinavian journal of gastroenterology. Supplement·2009
    Same journal

    Abstracts of the 39th Nordic Meeting of Gastroenterology, 30th Nordic Meeting of Digestive Endoscopy, 18th Nordic Meeting of Gastrointestinal Motility, and the Annual Endoscopy/Gastroenterology Nurses'/Assistants' Meeting Post-graduate course, 4-6 June 2008, Helsinki, Finland.

    Scandinavian journal of gastroenterology. Supplement·2008
    Same journal

    Abstracts from the XXXVIII Nordic Meeting of Gastroenterology, XXIX Nordic Meeting of Digestive Endoscopy, XVII Nordic Meeting of Gastrointestinal Motility, 6-9 June 2007, Reykjavik, Iceland.

    Scandinavian journal of gastroenterology. Supplement·2007
    Same journal

    Abstracts of the XXXVII Nordic Meeting of Gastroenterology, 3-5 May 2006, Vasteras, Sweden.

    Scandinavian journal of gastroenterology. Supplement·2006
    Same journal

    Novel approaches in the outpatient care of patients with chronic inflammatory bowel disease.

    Scandinavian journal of gastroenterology. Supplement·2006
    See all related articles

    Researchers found that prostaglandin E2 (PGE2) levels in rat blood are affected by in vitro production. Proper precautions, like indomethacin infusion, are crucial for accurate measurements in physiological studies.

    Area of Science:

    • Biochemistry
    • Physiology

    Background:

    • Circulating prostaglandin levels are important for physiological regulation.
    • Accurate measurement of prostaglandin E2 (PGE2) is essential for understanding its role.

    Purpose of the Study:

    • To determine the arterio-venous difference in circulating prostaglandin levels.
    • To investigate the influence of in vitro prostaglandin production on measurement accuracy.

    Main Methods:

    • Isotope derivative technique applied to rat plasma for PGE2 measurement.
    • Infusion of indomethacin to inhibit in vitro prostaglandin production.
    • Radio-gas-liquid-chromatography for arachidonic acid plasma level determination.

    Main Results:

    • No arterio-venous difference in PGE2 was observed without precautions to avoid in vitro production.

    Related Experiment Videos

  • Indomethacin infusion revealed minimal peripheral PGE2 levels, with right atrial levels at 60 +/- 20 pg/ml.
  • Fasted rats showed significantly higher PGE2 levels without indomethacin (330 +/- 35 pg/ml) compared to fed rats (210 +/- 70 pg/ml), correlating with higher arachidonic acid levels.

    • Conclusions:

    • In vitro prostaglandin production significantly impacts circulating PGE2 measurements.
    • Indomethacin blockade is necessary for accurate assessment of physiological PGE2 levels.
    • Fasting may increase PGE2 levels in rats, potentially linked to arachidonic acid availability.