Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The mouse ribosomal gene terminator consists of three functionally separable sequence elements.

A Kuhn1, A Normann, I Bartsch

  • 1Institut für Biochemie der Universität Würburg, FRG.

The EMBO Journal
|May 1, 1988
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

"Optimal timing for RNA isolation: Recommendations for placenta sample collection under clinical conditions".

Placenta·2025
Same author

Kinematic Effects of Combined Subthalamic and Dorsolateral Nigral Deep Brain Stimulation in Parkinson's Disease.

Journal of Parkinson's disease·2024
Same author

Spatially multiplexed single-molecule translocations through a nanopore at controlled speeds.

Nature nanotechnology·2023
Same author

A patient with refractory high-grade B-cell lymphoma and rapid progression under CAR-T-cell therapy was successfully salvaged with inotuzumab- ozogamicin.

Leukemia & lymphoma·2022
Same author

Cell Type-Specific Decomposition of Gingival Tissue Transcriptomes.

Journal of dental research·2021
Same author

Pathogen-specific antibody profiles in patients with severe systemic infections.

European cells & materials·2020
Same journal

The TPR2 corepressor forms condensates with repressors to fine-tune growth and development in rice.

The EMBO journal·2026
Same journal

SenFlag gene signature identifies senescent cells in mouse and human tissues through a conserved core transcriptional program.

The EMBO journal·2026
Same journal

Scalable phosphotyrosine enrichment with SH2 superbinder enables deep profiling of EGF responses.

The EMBO journal·2026
Same journal

Essential nucleus-apical pole linkage maintains division fidelity during Plasmodium progeny formation.

The EMBO journal·2026
Same journal

From cell atlases to mechanisms: bridging scRNA-seq discovery with in vivo genetics.

The EMBO journal·2026
Same journal

Mitochondrial calcium regulates lipid metabolism by modulating tethering of mitochondria to lipid droplets.

The EMBO journal·2026
See all related articles

The Sal box sequence motif is sufficient for mouse pre-ribosomal RNA (pre-rRNA) transcription termination by RNA polymerase I. Flanking pyrimidine-rich sequences are crucial for efficient and accurate 3' end formation of pre-rRNA.

Area of Science:

  • Molecular Biology
  • Gene Regulation
  • RNA Processing

Background:

  • Accurate 3' end formation of precursor ribosomal RNA (pre-rRNA) is essential for proper gene expression.
  • Transcription termination by RNA polymerase I (pol I) is a critical regulatory step in rRNA synthesis.

Purpose of the Study:

  • To investigate the structural requirements for 3' end formation of mouse pre-rRNA.
  • To identify sequence elements and nuclear factors involved in pol I transcription termination.

Main Methods:

  • Cell-free transcription system
  • In vivo assays using transfected rDNA minigene constructs in 3T6 cells
  • Analysis of DNA-protein interactions

Main Results:

Related Experiment Videos

  • The 18-bp conserved 'Sal box' sequence motif is sufficient for pol I termination and nascent RNA release.
  • Pyrimidine-rich sequences flanking the 'Sal box' are required for efficient and correct pre-rRNA 3' end formation.
  • Upstream and downstream flanking regions are recognized by distinct nuclear factors, influencing termination efficiency and precise end site selection.

    • Conclusions:

    • The 'Sal box' acts as the primary termination signal for mouse pre-rRNA transcription by pol I.
    • Flanking sequences and associated nuclear factors play critical roles in modulating the efficiency and accuracy of termination, ensuring the production of authentic pre-rRNA termini.