Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA-seq03:21

RNA-seq

12.2K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
12.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Patient-derived resources for decoding and targeting brain metastases ecosystems.

EMBO molecular medicine·2026
Same author

Longitudinal changes in DNA methylation in IDH-mutant glioma fuel disease progression through altered cell state differentiation.

Nature genetics·2026
Same author

Publisher Correction: Tumor transcriptional state predicts survival in immune-checkpoint-blockade-treated glioblastoma.

Nature cancer·2026
Same author

Acquired genetic and cell-state changes in IDH-mutant glioma progression.

Nature·2026
Same author

Tumor transcriptional state predicts survival in immune-checkpoint-blockade-treated glioblastoma.

Nature cancer·2026
Same author

Single-cell analysis highlights the significance of malignant cell IFN/MHC-II for immunotherapy response in head and neck squamous cell carcinoma.

Cell reports. Medicine·2026

Related Experiment Video

Updated: Feb 21, 2026

Nuclei Isolation from Fresh Frozen Brain Tumors for Single-Nucleus RNA-seq and ATAC-seq
06:22

Nuclei Isolation from Fresh Frozen Brain Tumors for Single-Nucleus RNA-seq and ATAC-seq

Published on: August 25, 2020

13.6K

Dissecting human gliomas by single-cell RNA sequencing.

Itay Tirosh1, Mario L Suvà1,2,3

  • 1Broad Institute of Harvard and MIT, Cambridge, Massachusetts.

Neuro-Oncology
|October 11, 2017
PubMed
Summary

Single-cell analysis reveals the complex cellular diversity within diffuse gliomas, offering new insights into tumor biology and classification. This approach overcomes limitations of bulk profiling for understanding these incurable brain tumors.

Keywords:
diffuse gliomasheterogeneitysingle-cell RNA sequencing

More Related Videos

Laser Capture Microdissection of Glioma Subregions for Spatial and Molecular Characterization of Intratumoral Heterogeneity, Oncostreams, and Invasion
09:09

Laser Capture Microdissection of Glioma Subregions for Spatial and Molecular Characterization of Intratumoral Heterogeneity, Oncostreams, and Invasion

Published on: April 12, 2020

7.5K
Author Spotlight: Exploring Strategies for Successful Immune Response Against Tumors
05:58

Author Spotlight: Exploring Strategies for Successful Immune Response Against Tumors

Published on: August 16, 2024

3.9K

Related Experiment Videos

Last Updated: Feb 21, 2026

Nuclei Isolation from Fresh Frozen Brain Tumors for Single-Nucleus RNA-seq and ATAC-seq
06:22

Nuclei Isolation from Fresh Frozen Brain Tumors for Single-Nucleus RNA-seq and ATAC-seq

Published on: August 25, 2020

13.6K
Laser Capture Microdissection of Glioma Subregions for Spatial and Molecular Characterization of Intratumoral Heterogeneity, Oncostreams, and Invasion
09:09

Laser Capture Microdissection of Glioma Subregions for Spatial and Molecular Characterization of Intratumoral Heterogeneity, Oncostreams, and Invasion

Published on: April 12, 2020

7.5K
Author Spotlight: Exploring Strategies for Successful Immune Response Against Tumors
05:58

Author Spotlight: Exploring Strategies for Successful Immune Response Against Tumors

Published on: August 16, 2024

3.9K

Area of Science:

  • Neuro-oncology
  • Genomics
  • Transcriptomics

Background:

  • Diffuse gliomas are common, incurable primary brain tumors.
  • Tumor complexity arises from diverse genetic and nongenetic factors.
  • Bulk profiling masks cellular heterogeneity within tumors.

Purpose of the Study:

  • To review single-cell analysis studies in clinical glioma samples.
  • To highlight insights gained from RNA expression profiling at single-cell resolution.
  • To explore impacts on understanding glioma biology and classification.

Main Methods:

  • Review of initial studies using single-cell DNA and RNA profiling.
  • Focus on RNA expression profiling in clinical glioma samples.
  • Analysis of cellular resolution data.

Main Results:

  • Single-cell studies provide new insights into glioma biology.
  • Revealed tumor heterogeneity and cancer cell lineages.
  • Identified cancer stem cell programs and tumor microenvironment characteristics.

Conclusions:

  • Single-cell analysis is crucial for understanding diffuse gliomas.
  • This approach enhances insights into tumor heterogeneity and biology.
  • Potential to improve glioma classification and treatment strategies.