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Updated: Feb 21, 2026

Monitoring Protein Adsorption with Solid-state Nanopores
Published on: December 2, 2011
Concentration-dependent protein adsorption at the nano-bio interfaces of polymeric nanoparticles and serum proteins
Tian-Xu Zhang1, Guan-Yin Zhu1, Bo-Yao Lu1
1State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
Aim:
A comprehensive understanding of nanoparticle (NP)-protein interaction (protein corona formation) is required. So far, many factors influencing this interaction have been investigated, like size and ζ potential. However, NPs exposure concentration has always been ignored. Herein, we aim to disclose the correlation of NPs exposure concentration with protein adsorption.
Materials & Methods:
Four polymeric NPs systems possessing similar sizes (230 ± 20 nm) but varied ζ potentials (-30 ∼ +40 mv) were prepared. Physicochemical properties and protein adsorption upon NP-protein interaction were characterized.
Results:
Protein adsorption capacity and adsorbed protein types were NPs concentration-dependent.
Conclusion:
Considering the critical impacts of protein adsorption on NPs delivery, our work could be an urgent warning about the possible risks of dosage adjustment of nanoformulations.
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