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Related Experiment Videos

Clinical application of Percoll gradient-separated bone marrow.

S Jagannath1, C L Reading, K A Dicke

  • 1Department of Hematology, University of Texas M.D. Anderson Hospital, Houston 77030.

Bone Marrow Transplantation
|February 1, 1987
PubMed
Summary
This summary is machine-generated.

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Bone marrow stem cell concentrates, free of platelets and granulocytes, effectively support hematopoietic restoration after transplants. Higher doses of colony-forming cells accelerated recovery, though graft-versus-host disease occurred in some patients.

Area of Science:

  • Hematology
  • Stem Cell Biology
  • Transplantation Medicine

Background:

  • Bone marrow stem cell concentrates are crucial for hematopoietic restoration.
  • Traditional buffy coat preparations suffer from clumping and cell loss.
  • A refined isolation technique is needed for improved stem cell transplantation.

Purpose of the Study:

  • To evaluate the transplantation potential of bone marrow stem cell concentrates.
  • To assess the hematopoietic restoration capacity of these enriched stem cells.
  • To determine the relationship between infused cell dose and patient recovery.

Main Methods:

  • Bone marrow stem cells were isolated from the 40/60% interface of Percoll gradients.
  • The mononuclear fraction, enriched in colony-forming cells (GM-CFC, BFU-E, GEMM-CFC), was used.

Related Experiment Videos

  • Hematopoietic restoration was evaluated in 14 patients undergoing allogeneic bone marrow transplantation.

    • Main Results:

    • The isolated fraction contained the majority of stem cells in <10% of the original cell number.
    • An inverse relationship was observed between infused GM-CFC dose and recovery time for granulocytes and platelets.
    • Recovery occurred within 10-15 days when >10^5 GM-CFC/kg were transplanted.
    • Seven patients developed acute graft-versus-host disease.

    Conclusions:

    • Bone marrow stem cell concentrates isolated via Percoll gradients offer a viable alternative to buffy coat preparations.
    • Infusion of sufficient GM-CFC doses (>10^5/kg) accelerates hematopoietic recovery post-transplant.
    • Further research is needed to mitigate graft-versus-host disease risks associated with T-lymphocyte content.