Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bioactive Silages from Agro-Industrial By-Products Based on Grape Pomace or Olive Mill Wastewater for Ruminants: Evolution of Phenolic Profiles, Antioxidant Activity, and Fatty Acid Composition.

Antioxidants (Basel, Switzerland)·2026
Same author

Comparative Evaluation of Functional Properties of Cow, Goat, and Donkey Milks Fermented with Lactic Acid Bacteria.

Antioxidants (Basel, Switzerland)·2025
Same author

Effects of mixing disbudded and horned young bulls during rearing on the post-mortem Longissimus thoracis muscle proteome.

Meat science·2025
Same author

Proteomic insights into broiler stress responses to LED lighting: Effects on liver proteome under neutral, cool, and warm spectra.

PloS one·2025
Same author

Antioxidant Synergy in a Mixture of Powder Plant Leaves and Effects on Metabolic Profile, Oxidative Status and Intestinal Morpho-Histochemical Features of Laying Hens.

Animals : an open access journal from MDPI·2025
Same author

Impact of UV pre-treatment on the Longissimus thoracis et lumborum muscle proteomes of dry-aged beef cuts: A characterisation within two sampling locations.

Meat science·2024

Related Experiment Video

Updated: Feb 21, 2026

Isolation of Intact, Whole Mouse Mammary Glands for Analysis of Extracellular Matrix Expression and Gland Morphology
12:49

Isolation of Intact, Whole Mouse Mammary Glands for Analysis of Extracellular Matrix Expression and Gland Morphology

Published on: October 30, 2017

18.0K

DIGE Analysis of Animal Tissues.

Alessio Di Luca1, Ruth Hamill2, Anne Maria Mullen2

  • 1Department of Agricultural and Food Sciences, School of Agriculture and Veterinary Medicine, University of Bologna, Viale Fanin 46, 40127, Bologna, Italy.

Methods in Molecular Biology (Clifton, N.J.)
|October 12, 2017
PubMed
Summary

Two-dimensional difference gel electrophoresis (2D-DIGE) offers enhanced protein separation and quantification. This method accurately analyzes protein variations in complex mixtures like porcine muscle exudate for meat quality assessment.

Keywords:
2D-DIGECentrifugal dripCyDye DIGE fluorImage analysisInternal standardIsoelectric focusingMachine learning algorithmMass spectrometryPorcine muscle exudateSDS-PAGE

More Related Videos

Sampling Strategies and Processing of Biobank Tissue Samples from Porcine Biomedical Models
05:07

Sampling Strategies and Processing of Biobank Tissue Samples from Porcine Biomedical Models

Published on: March 6, 2018

16.3K
Preparation of Drosophila Larval and Pupal Testes for Analysis of Cell Division in Live, Intact Tissue
08:05

Preparation of Drosophila Larval and Pupal Testes for Analysis of Cell Division in Live, Intact Tissue

Published on: May 19, 2020

9.0K

Related Experiment Videos

Last Updated: Feb 21, 2026

Isolation of Intact, Whole Mouse Mammary Glands for Analysis of Extracellular Matrix Expression and Gland Morphology
12:49

Isolation of Intact, Whole Mouse Mammary Glands for Analysis of Extracellular Matrix Expression and Gland Morphology

Published on: October 30, 2017

18.0K
Sampling Strategies and Processing of Biobank Tissue Samples from Porcine Biomedical Models
05:07

Sampling Strategies and Processing of Biobank Tissue Samples from Porcine Biomedical Models

Published on: March 6, 2018

16.3K
Preparation of Drosophila Larval and Pupal Testes for Analysis of Cell Division in Live, Intact Tissue
08:05

Preparation of Drosophila Larval and Pupal Testes for Analysis of Cell Division in Live, Intact Tissue

Published on: May 19, 2020

9.0K

Area of Science:

  • Proteomics
  • Biochemistry
  • Meat Science

Background:

  • Conventional 2D polyacrylamide gel electrophoresis (2D-PAGE) has limitations in sensitivity and reproducibility.
  • Two-dimensional difference gel electrophoresis (2D-DIGE) was developed to overcome drawbacks of 2D-PAGE.
  • 2D-DIGE utilizes direct fluorescent labeling of proteins for improved analysis.

Purpose of the Study:

  • To describe the application of 2D-DIGE for protein separation and quantification.
  • To analyze protein mixtures from porcine muscle exudate.
  • To characterize meat quality parameters using proteomic analysis.

Main Methods:

  • Proteins are labeled with isobaric fluorescent dyes (CyDyes) prior to isoelectric focusing (IEF).
  • Separation involves IEF in the first dimension and SDS-PAGE in the second dimension.
  • Quantification is achieved by imaging fluorescent dyes and normalizing spot intensities to an internal standard.

Main Results:

  • 2D-DIGE provides improved sensitivity and reduced experimental variation compared to 2D-PAGE.
  • Accurate within-gel matching allows for reliable comparison of protein expression levels.
  • Protein variations in porcine muscle exudate were effectively quantified.

Conclusions:

  • 2D-DIGE is a robust technique for quantitative proteomic analysis of complex biological samples.
  • The methodology is suitable for characterizing meat quality parameters.
  • Further analysis of identified proteins can be performed using mass spectrometry and other biochemical methods.