Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genome sequences of two isolates of the yeast <i>Candida zeylanoides</i>: UCD849 from soil in Ireland, and AWD from an African wild dog.

Microbiology resource announcements·2024
Same author

Femoral Arterial Doppler Use During Active Cardiopulmonary Resuscitation.

Annals of emergency medicine·2023
Same author

A Novel Point-of-care Ultrasound Curriculum for Air Critical Care Personnel.

The western journal of emergency medicine·2023
Same author

Elucidating Cellular Metabolism and Protein Difference Data from DIGE Proteomics Experiments Using Enzyme Assays.

Methods in molecular biology (Clifton, N.J.)·2022
Same author

Enzyme Assay Methods to Validate DIGE Proteomics Data.

Methods in molecular biology (Clifton, N.J.)·2022
Same author

Elucidating Cellular Metabolism and Protein Difference Data from DIGE Proteomics Experiments Using Enzyme Assays.

Methods in molecular biology (Clifton, N.J.)·2017

Related Experiment Video

Updated: Feb 21, 2026

Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry
11:54

Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry

Published on: March 23, 2020

10.4K

Enzyme Assay Methods to Validate DIGE Proteomics Data.

Andrew Dowd1

  • 1Monaghan Biosciences, Tyholland, Co. Monaghan, Ireland. Andrew.Dowd@monaghanbio.com.

Methods in Molecular Biology (Clifton, N.J.)
|October 12, 2017
PubMed
Summary

Enzyme activity assays, like those for superoxide dismutase and NADH dehydrogenase, can validate findings from Difference Gel Electrophoresis (DIGE) proteomic studies. This paper details these enzyme assays as practical examples for corroborating DIGE results.

Keywords:
DIGEDifference gel electrophoresisEnzymeEnzyme assayEnzymologyFluorescence spectrophotometryNADH dehydrogenaseSpectrophotometrySuperoxide dismutase

More Related Videos

Consensus Brain-derived Protein, Extraction Protocol for the Study of Human and Murine Brain Proteome Using Both 2D-DIGE and Mini 2DE Immunoblotting
10:51

Consensus Brain-derived Protein, Extraction Protocol for the Study of Human and Murine Brain Proteome Using Both 2D-DIGE and Mini 2DE Immunoblotting

Published on: April 10, 2014

16.8K
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

12.8K

Related Experiment Videos

Last Updated: Feb 21, 2026

Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry
11:54

Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry

Published on: March 23, 2020

10.4K
Consensus Brain-derived Protein, Extraction Protocol for the Study of Human and Murine Brain Proteome Using Both 2D-DIGE and Mini 2DE Immunoblotting
10:51

Consensus Brain-derived Protein, Extraction Protocol for the Study of Human and Murine Brain Proteome Using Both 2D-DIGE and Mini 2DE Immunoblotting

Published on: April 10, 2014

16.8K
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

12.8K

Area of Science:

  • Biochemistry
  • Proteomics

Background:

  • Difference Gel Electrophoresis (DIGE) is a powerful proteomics technique.
  • Validating proteomic data with orthogonal methods is crucial for scientific rigor.
  • Enzyme activity assays offer a complementary approach to proteomic analysis.

Purpose of the Study:

  • To demonstrate how enzyme activity assays can corroborate Difference Gel Electrophoresis (DIGE) proteomic results.
  • To provide detailed protocols for specific enzyme assays frequently used with DIGE.

Main Methods:

  • Detailed description of assay methods for superoxide dismutase activity.
  • Detailed description of assay methods for NADH dehydrogenase activity.
  • These assays are presented as examples for validating DIGE findings.

Main Results:

  • The paper outlines specific protocols for enzyme activity assays.
  • These methods are shown to be applicable for corroborating DIGE experiments.
  • The chosen enzyme assays are commonly employed in conjunction with DIGE.

Conclusions:

  • Enzyme activity assays serve as a valuable tool for validating proteomic data obtained from DIGE.
  • The detailed protocols facilitate the implementation of these corroborative methods.
  • Integrating enzyme assays enhances the reliability of proteomic findings.