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DR beta Taq I restriction fragment length polymorphism (RFLP) associated with insulin dependent diabetes mellitus
T W Hodge1, B O Barger, R T Acton
1Department of Microbiology, School of Medicine, University of Alabama, Birmingham 35294.
Disease Markers
|June 1, 1987
Abstract:
DNA from insulin-dependent diabetes mellitus patients (IDDM) and healthy control individuals was evaluated using Southern blotting to determine if a disease-associated restriction fragment length polymorphism could be found. Using a DR beta-cDNA probe hybridized to Taq I digested genomic DNA, a 3.7 kb fragment was found in all IDDM patients examined (n = 33). Although a high percentage (47 per cent) of the control population also carried the fragment, nearly one-third of those persons were serotyped as DR2. The possible role of gene regulation in the development of IDDM is discussed.