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Updated: May 9, 2026

Isolation and Expansion of the Adult Mouse Neural Stem Cells Using the Neurosphere Assay

Isolation and Expansion of the Adult Mouse Neural Stem Cells Using the Neurosphere Assay

Published on: November 20, 2010

Establishment of mouse expanded potential stem cells.

Jian Yang¹, David J Ryan¹, Wei Wang¹

¹Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1HH, UK.

|October 12, 2017

Summary

This summary is machine-generated.

Researchers created expanded potential stem cells (EPSCs) from mouse blastomeres and other stem cells. These EPSCs can contribute to all embryonic and extra-embryonic lineages, a significant advancement in developmental biology.

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Related Experiment Videos

Last Updated: May 9, 2026

Isolation and Expansion of the Adult Mouse Neural Stem Cells Using the Neurosphere Assay

Isolation and Expansion of the Adult Mouse Neural Stem Cells Using the Neurosphere Assay

Published on: November 20, 2010

Establishing Embryonic Mouse Neural Stem Cell Culture Using the Neurosphere Assay

Establishing Embryonic Mouse Neural Stem Cell Culture Using the Neurosphere Assay

Published on: January 11, 2011

Generation of Mice Derived from Induced Pluripotent Stem Cells

Generation of Mice Derived from Induced Pluripotent Stem Cells

Published on: November 29, 2012

Area of Science:

Developmental Biology
Stem Cell Biology
Epigenetics

Background:

Embryonic stem cells (ESCs) contribute to somatic and germline lineages but not extra-embryonic tissues.
Extra-embryonic tissues, like trophectoderm and primitive endoderm, are crucial for embryonic development.
Understanding stem cell potential is key to regenerative medicine and developmental studies.

Purpose of the Study:

To establish and characterize a novel stem cell type with broader developmental potential.
To investigate the contribution of these new stem cells to all embryonic lineages.
To explore the derivation of extra-embryonic lineages from these versatile stem cells.

Main Methods:

Generation of expanded potential stem cells (EPSCs) from eight-cell blastomeres, ESCs, and induced pluripotent stem cells (iPSCs).
Chimaera assays to assess the developmental contribution of single EPSCs.
In vitro derivation of trophoblast stem cells and extra-embryonic endoderm stem cells from EPSCs.
Epigenomic and single-cell transcriptomic analyses.

Main Results:

EPSCs were successfully established from various sources, including single blastomeres.
A single EPSC demonstrated the capacity to contribute to both the embryo proper and trophectoderm lineages.
Bona fide trophoblast stem cell and extra-embryonic endoderm stem cell lines were derived from EPSCs.
EPSCs exhibit blastomere-specific signatures and a dynamic DNA methylome.

Conclusions:

Expanded potential stem cells represent a unique cell type with totipotent-like properties in early development.
EPSCs offer a powerful model for studying early mammalian development and lineage specification.
The successful generation of mouse EPSCs suggests feasibility for establishing similar cells in other mammalian species.