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Enhanced α-amylase production by a marine protist, Ulkenia sp. using response surface methodology and genetic
Priyanka V Shirodkar1, Usha Devi Muraleedharan1
1a Department of Biotechnology , Goa University , Goa , India.
Preparative Biochemistry & Biotechnology
|October 12, 2017
Summary
Marine protists, thraustochytrids, were optimized for enhanced alpha-amylase production. Statistical methods including response surface methodology and genetic algorithms improved enzyme yield by 1.2-fold.
Area of Science:
- Biotechnology
- Marine Microbiology
Background:
- Amylases are crucial industrial enzymes.
- Marine protists, thraustochytrids, offer a novel source for enzyme production.
Purpose of the Study:
- To optimize alpha-amylase production from marine thraustochytrids.
- To identify key nutritional variables influencing enzyme yield and growth.
Main Methods:
- Utilized statistical experimental designs: response surface methodology (RSM) and genetic algorithm (GA).
- Employed a central composite design to assess interactive effects of glucose, corn starch, and yeast extract.
- Optimized Ulkenia sp. AH-2 growth and alpha-amylase activity.
Main Results:
- Maximized alpha-amylase activity reached 71.20 U/mL (GA) and 71.93 U/mL (RSM), closely matching the predicted 72.37 U/mL.
- Optimal growth achieved was 1.89A660nm.
- The optimized medium resulted in a 1.2-fold increase in alpha-amylase production.
Conclusions:
- Statistical optimization significantly enhances alpha-amylase production in marine thraustochytrids.
- RSM and GA are effective tools for optimizing bioprocesses in marine microorganisms.
- This study establishes a foundation for industrial-scale enzyme production from thraustochytrids.
