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Determination of Total Biotin by Liquid Chromatography Coupled with Immunoaffinity Column Cleanup Extraction:
George Joseph1, Ranjani Devi1, Elaine C Marley2
1AsureQuality Ltd, PO Box 41, Shortland St, Auckland 1140, New Zealand.
Journal of AOAC International
|October 13, 2017
Summary
A new method accurately determines total biotin in infant and adult formulas. This cost-effective LC-based technique, using immunoaffinity column cleanup, is suitable for global laboratory use and dispute resolution.
Area of Science:
- Analytical Chemistry
- Food Science
- Nutritional Analysis
Background:
- Accurate determination of total biotin is crucial for infant and adult nutritional formulas.
- Existing methods may lack the required selectivity, accuracy, or precision for reliable dispute resolution.
- Standard Method Performance Requirements (SMPR 2014.005) outline criteria for validated analytical methods.
Purpose of the Study:
- To develop and validate a candidate reference method for total biotin determination in various formula types.
- To assess the method's suitability for dispute resolution.
- To confirm the method meets AOAC Standard Method Performance Requirement 2014.005.
Main Methods:
- Liquid chromatography (LC) coupled with immunoaffinity column cleanup extraction.
- Single- and multilaboratory testing to evaluate performance.
- Application to diverse infant formula and adult nutritional matrices.
Main Results:
- The method demonstrated high selectivity, accuracy, and precision.
- Performance met the criteria outlined in AOAC SMPR 2014.005.
- Acceptable precision and accuracy were established across different formula types.
Conclusions:
- The developed LC method with immunoaffinity cleanup is a robust candidate reference method for total biotin analysis.
- The method is cost-effective, accessible for global use, and suitable for dispute resolution.
- Immunoaffinity column cleanup is identified as a critical factor for analytical success.
