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Association between ankylosing spondylitis and a 9.2 kb Pvu II class I HLA DNA restriction fragment: a reassessment

J P Durand1, J D Taurog

  • 1Harold C. Simmons Arthritis Research Center, University of Texas Southwestern Medical Center, Dallas 75235.

The Journal of Rheumatology
|July 1, 1988
PubMed

Insights

This study could not confirm a link between ankylosing spondylitis (AS) and a specific DNA fragment. Previous findings of this association in patients with AS were not replicated in our investigation.

Area of Science:

  • Genetics
  • Immunology
  • Rheumatology

Background:

  • Ankylosing spondylitis (AS) is a chronic inflammatory disease.
  • A previous study suggested an association between AS and a specific DNA restriction fragment (9.2 kb) linked to HLA-B7.

Purpose of the Study:

  • To investigate and attempt to replicate the reported association between ankylosing spondylitis and a specific HLA-B7 related DNA restriction fragment.

Main Methods:

  • Analysis of Pvu II digested genomic DNA using an HLA-B7 cDNA probe.
  • Comparison of the frequency of a 9.2 kb restriction fragment in patients with AS and healthy controls.

Main Results:

  • The 9.2 kb restriction fragment was detected in 10 of 33 ankylosing spondylitis patients.
  • The fragment was found in 17 of 41 controls.
  • These frequencies did not support the previously reported association.

Conclusions:

  • The study failed to confirm the association between ankylosing spondylitis and the specific 9.2 kb DNA restriction fragment identified by McDaniel et al.
  • Further research is needed to clarify genetic factors in ankylosing spondylitis.

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