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Probiotics for experimental obesity prevention: focus on strain dependence and viability of composition
Nazarii Kobyliak1, Tetyana Falalyeyeva, Tetyana Beregova
1Bogomolets National Medical University. nazariikobyliak@gmail.com.
Insights
Multistrain probiotics significantly reduced obesity prevalence and improved lipid profiles in rats compared to single strains. Live multistrain probiotics demonstrated superior efficacy in managing obesity-related metabolic changes.
Area of Science:
- Microbiology
- Animal Nutrition
- Metabolic Research
Background:
- Obesity is a growing health concern with complex etiologies.
- Monosodium glutamate (MSG) administration in neonates is a model for inducing obesity in animal studies.
- Probiotics are being investigated for their potential role in managing obesity and metabolic disorders.
Purpose of the Study:
- To comparatively evaluate the efficacy of single-strain, three-strain mix, and live multi-strain probiotics in an animal model of obesity.
- To assess the impact of different probiotic formulations on obesity prevalence, visceral adipose tissue (VAT) weight, and serum lipid levels.
Main Methods:
- A rat model of obesity was established using neonatal MSG injection.
- Rats were divided into seven groups, including a control, MSG-obesity group, and groups treated with lyophilized single-strain probiotics, a three-strain mix, or a live multi-strain probiotic (Symbiter).
- Efficacy was assessed by monitoring obesity prevalence, body weight, food consumption, VAT weight, and serum lipid levels.
Main Results:
- Neonatal MSG treatment induced obesity in rats.
- Probiotic administration, particularly live multi-strain formulations, significantly reduced obesity prevalence compared to single-strain probiotics.
- Multi-strain probiotics led to greater reductions in VAT weight and serum lipid levels than single-strain probiotics, with L. casei IMVB-7280 showing a trend towards stronger hypolipidemic effects.
Conclusions:
- Live multi-strain probiotics exhibit superior efficacy in combating obesity and associated metabolic dysregulation compared to single-strain probiotics.
- The synergistic interactions among multiple probiotic strains contribute to enhanced health benefits.
- Probiotic interventions hold promise for managing obesity and improving metabolic health.
Introduction:
a comparative animal study of the efficacy of intermittent short-course administration of lyophilised single-, three-, and live multistrain probiotic on obesity.
Methods:
We included 70 rats divided into seven groups (n = 10 in each). Rats of group I were intact. Newborn rats of groups II-VII were injected with monosodium glutamate (MSG) (4 mg/g). Rats of group II (MSG-obesity group) were untreated. The group III-V received lyophilised monoprobiotics B. animalis VKL, B. animalis VKB, and L. casei IMVB-7280, respectively. Group VI received the mix of these three probiotic strains. Group VII was treated with multiprobiotic "Symbiter", which contains 14 live probiotic strains (Lactobacillus, Bifi-dobacterium, Propionibacterium, Acetobacter genera).
Results:
Neonatal treatment with MSG caused stunted growth, which is why, despite the lack of weight gain dynamics and absence of significant food consumption rate and body weight changes at day 120, we noted the development of obesity in all MSG-obesity rats and in up to 20-70% after probiotic administration. Supplementation of probiotic composition, with preference to live strains, led to a significantly lower prevalence of obesity, and reduction of VAT weight and serum lipid levels as compared to single-strain probiotic. In our comparative single-strain analysis a trend towards more pronounced hypolipidaemic effect and VAT weight reduction was observed for lyophilised L. casei IMVB-7280 as compared to B. animalis VKL and VKB strains.
Conclusions:
Multistrain formed mutualistic interactions in mixtures and therefore able to share with different metabolites, affect differ-ent receptors and produced various of biologically active compounds which synergistic overall effect greater than the sum of the single effects.