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Related Experiment Video

Updated: Jul 17, 2026

Procedure for Lung Engineering
12:50

Procedure for Lung Engineering

Published on: March 8, 2011

Bioengineered lungs generated from human iPSCs-derived epithelial cells on native extracellular matrix.

Mahboobe Ghaedi1,2, Andrew V Le1,2, Go Hatachi1,2

  • 1Department of Anesthesiology, Yale University, New Haven, CT, USA.

Journal of Tissue Engineering and Regenerative Medicine
|October 13, 2017
PubMed
Summary

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Generating engineered lungs from stem cells is a promising step for lung transplantation. Human induced pluripotent stem cells (hiPSCs) can create lung progenitor cells to regenerate lung tissue.

Area of Science:

  • Regenerative Medicine
  • Bioengineering
  • Stem Cell Biology

Background:

  • Lung transplantation faces donor shortages, necessitating alternative strategies.
  • Decellularized lungs offer potential scaffolds for tissue engineering.
  • Challenges include sourcing reliable cells and scaling up for recellularization.

Purpose of the Study:

  • To investigate the regeneration of human lung tissue from stem cells in vitro.
  • To assess the feasibility of engineering lungs using induced pluripotent stem cell-derived cells.

Main Methods:

  • Generated lung progenitor cells from human induced pluripotent stem cells (hiPSCs).
  • Repopulated decellularized human and rat lungs with hiPSC-derived epithelial progenitor cells.
  • Utilized a lung bioreactor system for cell culture and assessment.
Keywords:
decellularized lungepithelial cellsinduced pluripotent stem cell (iPSC)lung implanttissue regeneration bioreactor

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Related Experiment Videos

Last Updated: Jul 17, 2026

Procedure for Lung Engineering
12:50

Procedure for Lung Engineering

Published on: March 8, 2011

Engineered Lung Tissues Prepared from Decellularized Lung Slices
08:01

Engineered Lung Tissues Prepared from Decellularized Lung Slices

Published on: January 21, 2022

Imaging-Guided Bioreactor for Generating Bioengineered Airway Tissue
11:01

Imaging-Guided Bioreactor for Generating Bioengineered Airway Tissue

Published on: April 6, 2022

Main Results:

  • hiPSC-derived epithelial progenitor cells successfully lined decellularized human lungs.
  • These cells expressed key epithelial markers in the bioreactor system.
  • Human cells attached and proliferated in decellularized rat lungs, similar to human lung matrix.

Conclusions:

  • Repopulating lung matrix with hiPSC-derived epithelial cells is a viable strategy for human lung regeneration.
  • This research represents a significant early step toward the clinical translation of engineered lungs.