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Conventional and real time RT-PCR assays for the detection and differentiation of variant rabbit hemorrhagic disease

K P Dalton1, J L Arnal2, A A Benito2

  • 1Instituto Universitario de Biotecnología de Asturias, Universidad de Oviedo, Oviedo, Spain.

Journal of Virological Methods
|October 13, 2017
PubMed

Keywords:
Iberian peninsulaRHDVbRT-qPCRRecombinantVariant RHDVYoung rabbits

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Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...