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Related Concept Videos

Reproductive Cloning01:27

Reproductive Cloning

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Reproductive cloning is the process of producing a genetically identical copy—a clone—of an entire organism. While clones can be produced by splitting an early embryo—similar to what happens naturally with identical twins—cloning of adult animals is usually done by a process called somatic cell nuclear transfer (SCNT).
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic...
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The first successfully cloned mammal was Dolly, a sheep, born on 5th July 1996 at Roslin Institute, Scotland. The cloned sheep was named after the American singer Dolly Parton. Dolly lived for seven years and died of respiratory complications, which is speculated to be due to the actual age of her DNA. Because the DNA in cloned cells belongs to an older individual,  the cloned individual’s life expectancy may be affected. Indeed, analysis of Dolly’s DNA revealed shorter...
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Related Experiment Video

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A Microfluidic Platform for High-throughput Single-cell Isolation and Culture
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Highly efficient cellular cloning using Ferro-core Micropallet Arrays.

Trisha M Westerhof1, Wesley A Cox-Muranami2, Guann-Pyng Li2

  • 1School of Medicine, Department of Medicine, Division of Hematology/Oncology, University of California, Irvine, United States.

Scientific Reports
|October 14, 2017
PubMed
Summary
This summary is machine-generated.

Researchers developed a new ferro-core micropallet array for faster, more efficient cell cloning. This innovation accelerates the isolation of genetically modified cells, advancing biological research.

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Area of Science:

  • Cellular Biology
  • Molecular Biology
  • Biotechnology

Background:

  • Genetic manipulation is crucial for biological research but relies on slow, labor-intensive cloning methods.
  • Existing cell cloning techniques often take months and require multiple purification steps.

Purpose of the Study:

  • To adapt a novel ferro-core micropallet array platform for rapid isolation of clonal cell populations.
  • To improve the efficiency and speed of obtaining genetically manipulated adherent cells.

Main Methods:

  • Utilized a ferro-core micropallet array platform for isolating adherent cells.
  • Employed laser release and magnetic recovery of single micropallets containing single cells.
  • Used transfected rat 208F cells with oncogene or fluorescent reporter constructs.

Main Results:

  • Successfully isolated and expanded pure populations of genetically manipulated cells.
  • Demonstrated the platform's ability to use various cytometric parameters for cell identification.
  • Achieved significant acceleration in obtaining clonally expanded cell colonies.

Conclusions:

  • The ferro-core micropallet array platform offers a faster, more efficient alternative to traditional cell cloning methods.
  • This technology can be broadly applied in cancer, developmental, and stem cell biology.
  • The platform reduces the need for repetitive purification, increasing research throughput.