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Characterization and genomic study of "phiKMV-Like" phage PAXYB1 infecting Pseudomonas aeruginosa

Xinyan Yu1, Yue Xu1, Yu Gu1

  • 1Key Laboratory of Pathogen Biology of Jiangsu Province, Department of Microbiology, Nanjing Medical University, Nanjing, 211166, China.

Scientific Reports
|October 14, 2017
PubMed

Insights

A novel bacteriophage, PAXYB1, effectively lyses Pseudomonas aeruginosa, including clinical strains. This virulent phage shows potential for developing biological disinfectants against P. aeruginosa infections.

Area of Science:

  • Microbiology
  • Virology
  • Genomics

Background:

  • Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic pathogen causing significant infections.
  • Bacteriophages are viruses that infect bacteria and are being explored as therapeutic agents.
  • Wastewater is a rich source for isolating novel bacteriophages with lytic activity.

Purpose of the Study:

  • To characterize the novel bacteriophage PAXYB1 isolated from wastewater.
  • To determine its lytic activity against clinical isolates of P. aeruginosa.
  • To analyze the complete genome sequence of PAXYB1.

Main Methods:

  • Morphological characterization using electron microscopy.
  • Lytic activity assays against P. aeruginosa strains.
  • Whole-genome sequencing and bioinformatic analysis.

Main Results:

  • PAXYB1 is a Podoviridae bacteriophage with a linear, double-stranded DNA genome of 43,337 bp.
  • The phage exhibits virulent properties and belongs to the phiKMV-like phages genus.
  • PAXYB1 efficiently lysed P. aeruginosa PAO1 and several clinical isolates.

Conclusions:

  • PAXYB1 is a newly identified lytic bacteriophage targeting P. aeruginosa.
  • Its genomic and lytic properties are elucidated.
  • PAXYB1 holds promise for use as a biological disinfectant to combat P. aeruginosa infections.

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