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[Isolation and purification of phospholipase C from Clostridium perfringens and antibodies against it using polymeric
Abstract:
A new polymeric biospecific adsorbent intended for isolation of Clostridium perfringens phospholipase C (PLC) and PLC-specific antibodies is discussed. It was obtained by radical copolymerization of acrylamide, methylenbisacrylamide, and the acryl acid chloranhydride-acylated substrate of PLC (chicken yolk lecithovitellin). Maximal adsorption of PLC was observed in the presence of the enzyme activator, and the highest amount of PLC was eluted in case of its minimal adsorption. The "residual" activity of PLC on the adsorbent was used to isolate homologous antibodies from the anti-perfringens serum. PLC and PLC-specific antibodies were serologically pure in the agar precipitation test with the anti-perfringens serum and the primary PLC concentrate, respectively; the antibodies gave only one zone in PAAG-electrophoresis.