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Chromatin Immunoprecipitation- ChIP

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...

Immunoprecipitation

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...

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Updated: Jun 29, 2026

Chromatin Isolation by RNA Purification (ChIRP)

Chromatin Isolation by RNA Purification (ChIRP)

Published on: March 25, 2012

Chromatin RNA Immunoprecipitation (ChRIP).

Tanmoy Mondal¹, Santhilal Subhash¹, Chandrasekhar Kanduri²

¹Department of Medical Biochemistry and Cell Biology, Institute of Biomedicine, The Sahlgrenska Academy, University of Gothenburg, Medicinaregatan 9A, Box 440, Gothenburg, 40530, Sweden.

Methods in Molecular Biology (Clifton, N.J.)

|October 14, 2017

Summary

Researchers explored long noncoding RNAs (lncRNAs) in chromatin organization. They developed chromatin RNA immunoprecipitation (ChRIP) to identify lncRNAs in active and inactive chromatin, advancing epigenetic code understanding.

Keywords:

Active chromatin Chromatin EZH2 Epigenetics Gene regulation H3K27me3 Inactive chromatin Long noncoding RNA

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Last Updated: Jun 29, 2026

Chromatin Isolation by RNA Purification (ChIRP)

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Published on: March 25, 2012

Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing (ChIP-seq)

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Area of Science:

Epigenetics and Molecular Biology
Chromatin organization and function
Long noncoding RNA (lncRNA) research

Background:

Growing interest in the functional roles of lncRNAs in organizing chromatin.
lncRNAs may act as intermediaries between chromatin and remodelers, influencing the epigenetic code.
The precise mechanisms by which lncRNAs target chromatin remodelers remain unclear.

Purpose of the Study:

To investigate the functional role of lncRNAs in chromatin organization.
To characterize lncRNAs associated with active and inactive chromatin compartments.
To elucidate how lncRNAs target chromatin remodelers to specific genomic locations.

Main Methods:

Development and optimization of chromatin RNA immunoprecipitation (ChRIP) technology.
Utilizing ChRIP to identify and analyze chromatin-bound lncRNAs.
Comparative analysis of lncRNAs in active versus inactive chromatin.

Main Results:

Successfully developed and optimized ChRIP technology for lncRNA analysis.
Identified specific lncRNAs associated with distinct chromatin compartments.
Provided a method to characterize chromatin-associated lncRNAs.

Conclusions:

ChRIP is an effective tool for identifying chromatin-bound lncRNAs.
This research enhances understanding of lncRNA functions in epigenetic modifications.
Further investigation into lncRNA-chromatin remodeler interactions is warranted.