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Related Concept Videos

Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
Immunoprecipitation01:20

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...

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Related Experiment Video

Updated: Jul 20, 2026

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin
24:02

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin

Published on: April 12, 2014

MOBE-ChIP: Probing Cell Type-Specific Binding Through Large-Scale Chromatin Immunoprecipitation.

Shenqi Wang1, On Sun Lau2

  • 1Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore, 117543, Singapore.

Methods in Molecular Biology (Clifton, N.J.)
|October 14, 2017
PubMed
Summary

Researchers developed Maximized Objects for Better Enrichment (MOBE)-ChIP, a sensitive method to identify DNA targets of cell type-specific transcription factors. This technique enhances chromatin immunoprecipitation (ChIP) signals for better gene regulation studies.

Keywords:
Cell type-specific transcriptional regulatorChIP-SeqChromatin immunoprecipitation (ChIP)MOBE-ChIPProtein–DNA interactions

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TChIP-Seq: Cell-Type-Specific Epigenome Profiling
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TChIP-Seq: Cell-Type-Specific Epigenome Profiling

Published on: January 23, 2019

Related Experiment Videos

Last Updated: Jul 20, 2026

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin
24:02

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin

Published on: April 12, 2014

Chromatin Immunoprecipitation (ChIP) in Mouse T-cell Lines
11:39

Chromatin Immunoprecipitation (ChIP) in Mouse T-cell Lines

Published on: June 17, 2017

TChIP-Seq: Cell-Type-Specific Epigenome Profiling
07:28

TChIP-Seq: Cell-Type-Specific Epigenome Profiling

Published on: January 23, 2019

Area of Science:

  • Molecular Biology
  • Genetics
  • Plant Science

Background:

  • Cell type-specific transcriptional regulators are vital for multicellular organisms.
  • Identifying their in vivo DNA targets genome-wide is challenging due to limited expression.
  • Current chromatin immunoprecipitation (ChIP) methods may lack sensitivity for these specific targets.

Purpose of the Study:

  • To develop a more sensitive ChIP technique for detecting cell type-specific transcription factor binding sites.
  • To overcome technical challenges in probing DNA targets of lowly expressed regulators.
  • To establish a robust method for genome-wide analysis of transcription factor-DNA interactions.

Main Methods:

  • Development of Maximized Objects for Better Enrichment (MOBE)-ChIP.
  • Performing ChIP at a substantially larger experimental scale.
  • Optimizing low background conditions for enhanced signal detection.
  • Application in the model plant Arabidopsis for transcription factor studies.

Main Results:

  • MOBE-ChIP significantly improves the sensitivity of detecting cell type-specific ChIP signals.
  • The method allows for more effective identification of transcription factor DNA targets.
  • Successful application demonstrated in the model plant Arabidopsis.

Conclusions:

  • MOBE-ChIP is a powerful strategy to enhance ChIP signal sensitivity.
  • The technique facilitates the study of gene regulation by cell type-specific transcription factors.
  • MOBE-ChIP is adaptable for various biological systems and general ChIP signal improvement.