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Updated: Feb 20, 2026
Preparation of Tunable Extracellular Matrix Microenvironments to Evaluate Schwann Cell Phenotype Specification
Published on: June 2, 2020
[Automatic morphometric analysis as a method for determining the level of extracellular matrix components and for
I S Gumenyuk1, L M Chuprinenko1, A S Sotnichenko1
1Kuban State Medical University, Ministry of Health of the Russian Federation, Krasnodar, Russia.
Abstract:
Automated image analysis methods are highly important for biotechnology research. The authors developed and tested a program for the morphometric analysis of photomicrographs of the sections processed using the standard immunohistochemical examination protocols. The color deconvolution method used in the algorithm was proven to be effective in mapping the distribution of DAB chromogen in the sample containing multiple dyes. The experiment demonstrated that the level of extracellular matrix proteins could be comparatively quantified in different groups of samples. The effective methods for the quantitative analysis of the Ki-67 labelling index were also tested using the same algorithms. The developed program was published under free GPL 3.0.
