Cu²⁺-Complex of hydrophilic nitrogen-rich polymer dots applied as a new MRI contrast agent

We report on a novel Cu²⁺-complex of nitrogen-rich polymer dots for magnetic resonance imaging (MRI). The N-rich polymer dots are prepared from N-vinyl imidazole (VIm) by a one-pot hydrothermal synthesis at 220 °C (24 h) and used later on to fabricate a Cu²⁺-PVIm dot complex via efficient incorporation of Cu²⁺ into aqueous medium. The obtained Cu²⁺-PVIm dot complexes display relaxivity (r₁ = 1.05 mM^-1 s^-1) two times higher than Cu²⁺ in aqueous solution (r₁ = 0.43 mM^-1 s^-1) and three times higher than Cu²⁺ in aqueous solution coordinated with VIm monomers (r₁ = 0.32 mM^-1 s^-1), which show a remarkable contrast enhancement for T₁-weighted MRI while efficiently labeling MCF-7 cells and other biomedical applications.