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Antitumor effect of miR-27b-3p on lung cancer cells via targeting Fzd7
1Department of Respiratory Medicine, Huangdao Division, The Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, China. sunyong12321@126.com.
Objective:
Lung cancer is the most common malignancy with the highest mortality rate among cancers. microRNAs (miRNAs) have been confirmed to be closely related to the physiological disorder, especially the tumor process. This study aimed to investigate the effect of miR-27b-3p on lung tumor cells.
Materials And Methods:
The expressions of miR-27b-3p in lung tumors and adjacent non-tumors lung tissues were compared. We test the bonding effect of miR-27b-3p on the Fzd7 promoter, and miR-27b-3p effects on the Fzd7 expression in both NCI-H446 and A549 cells. Then, effects of miR-27b-3p and Fzd7 on these cells viability, survival and apoptosis were detected, respectively. In addition, the possible mechanism of miR-27b-3p affected these cells apoptosis was explored by analyzing the expression of apoptosis-related factors.
Results:
We found that miR-27b-3p was low expressed in lung tumors compared to adjacent non-tumorous lung tissues. miR-27b-3p directly targeted Fzd7 promoter and negatively regulated Fzd7 expression. Fzd7 promoted NCI-H446 and A549 cells viability and survival, inhibited cells apoptosis. However, miR-27b-3p effects on these cells were quite the opposite to Fzd7. The expressions of apoptosis-related factors were associated positively with miR-27b-3p and showed a negative correlation with Fzd7 expression.
Conclusions:
The miR-27b-3p was lowly expressed in lung cancer tissues, and played the role of a tumor suppressor. It could promote cell apoptosis and suppress cancer cells viability and survival via down-regulating Fzd7. It suggested that miR-27b-3p might be a potential target for the prophylaxis and treatment of lung cancer.
Insights
MicroRNA-27b-3p is underexpressed in lung tumors and acts as a tumor suppressor. It promotes apoptosis and inhibits cancer cell growth by downregulating Fzd7, suggesting its potential as a lung cancer therapeutic target.
Area of Science:
- Oncology
- Molecular Biology
- Gene Regulation
Background:
- Lung cancer is a leading cause of cancer mortality worldwide.
- MicroRNAs (miRNAs) are implicated in cancer development and progression.
- The specific role of miR-27b-3p in lung tumorigenesis requires further elucidation.
Purpose of the Study:
- To investigate the expression levels of miR-27b-3p in lung tumors.
- To determine the regulatory effect of miR-27b-3p on Fzd7 expression.
- To explore the impact of miR-27b-3p and Fzd7 on lung cancer cell behavior and apoptosis.
Main Methods:
- Comparative analysis of miR-27b-3p expression in tumor and adjacent non-tumor lung tissues.
- Luciferase reporter assays to confirm miR-27b-3p binding to the Fzd7 promoter.
- Cell viability, survival, and apoptosis assays in lung cancer cell lines (NCI-H446, A549) following modulation of miR-27b-3p and Fzd7.
- Analysis of apoptosis-related gene expression.
Main Results:
- miR-27b-3p expression was significantly lower in lung tumors compared to non-tumor tissues.
- miR-27b-3p directly targets the Fzd7 promoter, leading to decreased Fzd7 expression.
- Fzd7 enhanced cancer cell viability and survival while inhibiting apoptosis; miR-27b-3p exerted opposite effects.
- Apoptosis-related factors correlated positively with miR-27b-3p and negatively with Fzd7.
Conclusions:
- miR-27b-3p functions as a tumor suppressor in lung cancer.
- Downregulation of Fzd7 by miR-27b-3p promotes apoptosis and inhibits cancer cell viability and survival.
- miR-27b-3p represents a potential therapeutic target for lung cancer treatment and prevention.
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