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Glutamine synthetase specific activity and protein concentration in symbiotic Anabaena associated with Azolla
K Y Lee1, C M Joseph, J C Meeks
1Dept. of Bacteriology, University of California, Davis 95616.
Abstract:
Glutamine synthetase (GS) is the primary NH4+ assimilating enzyme of cyanobacteria. The specific activities and cellular protein concentration of GS in symbiotic cyanobacteria associated with the water fern Azolla caroliniana were determined and compared to free-living cultures of Nostoc sp. strain 7801, a strain originally isolated from symbiotic association with the bryophyte Anthoceros punctatus. Both the in vitro specific activity and concentration of GS in symbiotic cyanobacteria separated from A. caroliniana were approximately 3-fold lower than the free-living Nostoc sp. strain 7801 culture. These results imply depressed synthesis of GS by the symbiont associated with A. caroliniana.