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Related Experiment Videos

A Window Into Clinical Next-Generation Sequencing-Based Oncology Testing Practices.

Rakesh Nagarajan, Angela N Bartley, Julia A Bridge

    Archives of Pathology & Laboratory Medicine
    |October 14, 2017
    PubMed
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    Clinical laboratories primarily use tumor-only targeted sequencing for cancer variant detection. While most employ desktop sequencers and commercial kits, diverse practices exist in next-generation sequencing oncology testing.

    Area of Science:

    • Oncology
    • Molecular Diagnostics
    • Clinical Laboratory Science

    Background:

    • Precision medicine relies on detecting acquired cancer variants.
    • Limited data exists on current clinical laboratory practices for oncology testing.
    • Understanding these practices is crucial for advancing cancer care.

    Purpose of the Study:

    • To analyze next-generation sequencing (NGS) oncology testing practices.
    • To report findings from College of American Pathologists proficiency testing surveys.
    • To identify trends and variations in molecular oncology testing.

    Main Methods:

    • Utilized proficiency testing survey data from over 250 laboratories performing molecular oncology tests.
    • Focused on data from 60 laboratories conducting NGS-based oncology testing.

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  • Examined specimen requirements and assay characteristics.

    • Main Results:

    • Most laboratories offer or plan to offer NGS-based oncology testing.
    • Predominant methods include tumor-only targeted sequencing for single-nucleotide variants and small insertions/deletions.
    • Desktop sequencers and predesigned commercial kits are commonly used, yet significant practice diversity exists.

    Conclusions:

    • Findings offer insights into current NGS oncology testing landscape.
    • Data can inform national discussions on clinical laboratory quality systems and regulation.
    • Supports data-driven precision oncology efforts and oversight.