Related Experiment Video
Updated: Feb 20, 2026

An Efficient Sample Preparation Method to Enhance Carbohydrate Ion Signals in Matrix-assisted Laser Desorption/Ionization Mass Spectrometry
Published on: July 29, 2018
Effect of metal surfaces on matrix-assisted laser desorption/ionization analyte peak intensities
Vidhyullatha Kancharla1, Sajid Bashir1,2, Jingbo L Liu2
11 Department of Chemistry, Texas A&M University-Kingsville, Kingsville, TX, USA.
Abstract:
Different metal surfaces in the form of transmission electron microscope grids were examined as support surfaces in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry with a view towards enhancement of peptide signal intensity. The observed enhancement between 5-fold and 20-fold relative to the normal stainless steel slide was investigated by applying the thermal desorption model for matrix-assisted laser desorption/ionization. A simple model evaluates the impact that the thermal properties of the metals have on the ion yield of the analyte. It was observed that there was not a direct, or strong, correlation between the thermal properties of the metals and the corresponding ion yield of the peptides. The effects of both fixed and variable laser irradiances versus ion yield were also examined for the respective metals studied. In all cases the use of transmission electron microscope grids required much lower laser irradiances in order to generate similar peak intensities as those observed with a stainless steel surface.
More Related Videos
06:56Characterization of Synthetic Polymers via Matrix Assisted Laser Desorption Ionization Time of Flight MALDI-TOF Mass Spectrometry
Published on: June 10, 2018
09:38Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer
Published on: November 26, 2013
Related Concept Videos
Matrix-Assisted Laser Desorption Ionization (MALDI)
MALDI-TOF Mass Spectrometry
Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences
Mass Analyzers: Overview
Mass Spectrum: Interpretation
Mass Spectrometry: Isotope Effect