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Updated: Feb 20, 2026
Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells
Published on: May 30, 2025
CRISPR/Cas9-mediated noncoding RNA editing in human cancers
Jie Yang1,2, Xiaodan Meng1,2, Jinchang Pan1,2
1a Department of Biochemistry and Molecular Biology , Medical School of Ningbo University , Ningbo , Zhejiang , China.
Abstract:
Cancer is characterized by multiple genetic and epigenetic alterations, including a higher prevalence of mutations of oncogenes and/or tumor suppressors. Mounting evidences have shown that noncoding RNAs (ncRNAs) are involved in the epigenetic regulation of cancer genes and their associated pathways. The clustered regularly interspaced short palindromic repeats (CRISPR)-associated nuclease 9 (CRISPR/Cas9) system, a revolutionary genome-editing technology, has shed light on ncRNA-based cancer therapy. Here, we briefly introduce the classifications and mechanisms of CRISPR/Cas9 system. Importantly, we mainly focused on the applications of CRISPR/Cas9 system as a molecular tool for ncRNA (microRNA, long noncoding RNA and circular RNA, etc.) editing in human cancers, and the novel techniques that are based on CRISPR/Cas9 system. Additionally, the off-target effects and the corresponding solutions as well as the challenges toward CRISPR/Cas9 were also evaluated and discussed. Long- and short-ncRNAs have been employed as targets in precision oncology, and CRISPR/Cas9-mediated ncRNA editing may provide an excellent way to cure cancer.
Insights
The clustered regularly interspaced short palindromic repeats (CRISPR)-associated nuclease 9 (CRISPR/Cas9) system offers a novel approach for cancer therapy by editing noncoding RNAs (ncRNAs). This technology shows promise for precision oncology and treating human cancers.
Area of Science:
- Genetics
- Molecular Biology
- Oncology
Background:
- Cancer involves genetic and epigenetic changes, with noncoding RNAs (ncRNAs) regulating cancer genes.
- The CRISPR/Cas9 system is a powerful genome-editing tool with potential in ncRNA-based cancer therapy.
Purpose of the Study:
- To review the CRISPR/Cas9 system's classifications, mechanisms, and applications in editing ncRNAs for cancer therapy.
- To discuss novel CRISPR/Cas9-based techniques, off-target effects, and challenges in cancer treatment.
Main Methods:
- Review of CRISPR/Cas9 system classifications and mechanisms.
- Focus on CRISPR/Cas9 applications for editing microRNAs, long noncoding RNAs, and circular RNAs in cancers.
- Evaluation of off-target effects and solutions.
Main Results:
- CRISPR/Cas9 system provides a versatile tool for precise ncRNA editing in cancer research.
- Novel techniques based on CRISPR/Cas9 are emerging for therapeutic applications.
- Understanding and mitigating off-target effects are crucial for clinical translation.
Conclusions:
- CRISPR/Cas9-mediated ncRNA editing represents a promising strategy for precision oncology.
- This technology holds potential for developing novel cancer therapies targeting long and short ncRNAs.
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