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EZH2 regulates dental pulp inflammation by direct effect on inflammatory factors
Tianqian Hui1, Peng A2, Yuan Zhao2
1State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan 610041, China; Department of Pediatric Dentistry, Peking University School and Hospital of Stomatology, Peking University, Beijing, China.
Archives of Oral Biology
|October 14, 2017
Summary
Enhancer of Zeste Homolog 2 (EZH2) promotes inflammation in human dental pulp cells. Down-regulating EZH2 reduces inflammatory responses, suggesting its key role in pulpitis pathogenesis.
Area of Science:
- Oral Biology
- Molecular Biology
- Immunology
Background:
- Pulpitis is a complex dental disease influenced by genetic and environmental factors.
- The role of epigenetic regulators like Enhancer of Zeste Homolog 2 (EZH2) in pulpitis is not fully understood.
Purpose of the Study:
- To investigate the function of EZH2 in the inflammatory response of human dental pulp cells (HDPCs) and dental pulp tissues.
- To elucidate the molecular mechanisms by which EZH2 influences pulpal inflammation.
Main Methods:
- Quantitative real-time PCR (q-PCR) and Luminex assay to measure inflammatory cytokine expression in HDPCs treated with EZH2 modulators.
- Histological analysis of dental pulp tissues in a rat pulpitis model.
- Chromatin immunoprecipitation (ChIP) assay to identify EZH2 binding sites on cytokine gene promoters.
Main Results:
- EZH2 down-regulation significantly inhibited the expression and secretion of inflammatory factors (IL-6, IL-8, IL-15, CCL2, CXCL12) in HDPCs.
- EZH2 silencing attenuated rhTNF-α-induced inflammatory factor promotion.
- EZH2 down-regulation reduced pulpal inflammation in a rat model.
- EZH2 directly binds to the promoters of IL-6, IL-8, and CCL2, influencing their transcription.
Conclusions:
- EZH2 plays a significant role in inducing inflammation in human dental pulp cells.
- EZH2 down-regulation effectively attenuates inflammatory responses in dental pulp.
- Targeting EZH2 may offer a therapeutic strategy for managing pulpitis.