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Serial-omics characterization of equine urine
Min Yuan1, Susanne B Breitkopf1,2, John M Asara1,2
1Division of Signal Transduction, Beth Israel Deaconess Medical Center, Boston, Massachusetts, United States of America.
Plos One
|October 14, 2017
Summary
This study presents a comprehensive mass spectrometry-based omics analysis of equine urine, identifying 46 proteins and 474 metabolites. These findings establish a baseline for healthy horse urine composition, aiding in health and drug monitoring.
Area of Science:
- Veterinary Medicine
- Biochemistry
- Analytical Chemistry
Background:
- Horse urine is a valuable source of biomarkers for health and disease.
- Mass spectrometry enables precise molecular analysis of biological samples.
- Comprehensive characterization of equine urine composition is essential for establishing health baselines.
Purpose of the Study:
- To perform a serial-omics characterization of equine urine using mass spectrometry.
- To identify and quantify proteins, polar metabolites, and non-polar lipids in horse urine.
- To establish a baseline profile of healthy mare urine composition.
Main Methods:
- Equine urine was subjected to liquid-liquid extraction using methyl tert-butyl ether (MTBE) and methanol.
- High-performance liquid chromatography-high resolution tandem mass spectrometry (LC-MS/MS) was employed for analysis.
- Proteins, polar metabolites, and non-polar lipids were purified, identified, and quantified from microliter-level urine samples.
Main Results:
- 46 urine proteins were identified, including those related to kidney function and circulation.
- 474 small polar metabolites were detected, primarily linked to urea cycle, amino acid pathways, and diet.
- Only 10 lipid molecules, mainly triglycerides and phospholipids, were identified.
Conclusions:
- A complete mass spectrometry-based omics characterization of equine urine was achieved from a small sample volume.
- The established baseline data are crucial for monitoring horse health, disease progression, and drug use.
- This study demonstrates the utility of equine urine as a non-invasive diagnostic fluid.