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Related Experiment Videos

Cell cycle specific radiosensitisation by the disulfiram and copper complex.

Mathias Tesson1, Giorgio Anselmi2, Caitlin Bell3

  • 1Radiation Oncology, Institute of Cancer Sciences, Wolfson Wohl Translational Cancer Research Center, University of Glasgow, Bearsden, Glasgow, UK.

Oncotarget
|October 15, 2017
PubMed
Summary

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Disulfiram and copper complex (DSF:Cu) shows potential as a radiosensitizing anti-cancer agent. It enhances radiation therapy effectiveness, particularly when combined with gemcitabine, suggesting a promising combination treatment for cancer patients.

Area of Science:

  • Oncology
  • Radiotherapy
  • Cancer Cell Biology

Background:

  • Disulfiram and copper complex (DSF:Cu) is a potent radiosensitizing agent.
  • Copper stabilizes disulfiram (DSF) and inhibits DNA replication enzymes, prompting investigation into its cell cycle effects.

Purpose of the Study:

  • To investigate the effect of DSF:Cu on cell cycle regulation in cancer cells.
  • To evaluate the radiosensitizing efficacy of DSF:Cu, alone and in combination with gemcitabine.

Main Methods:

  • Flow cytometry and immunoblotting were used to assess cell cycle progression in SK-N-BE(2c) and UVW cell lines.
  • Clonogenic assays determined the radiosensitizing potency of DSF:Cu and gemcitabine.

Main Results:

Keywords:
DNA replicationcopperdisulfiramgemcitabineradiosensitisation

Related Experiment Videos

  • DSF:Cu inhibited DNA synthesis in neuroblastoma and glioma cells.
  • DSF:Cu significantly enhanced radiation kill, with dose enhancement factors of 2.64 and 2.84.
  • Combination with gemcitabine potentiated DSF:Cu-induced radiosensitization, with efficacy dependent on treatment schedule.

    • Conclusions:

    • DSF:Cu exhibits cell cycle-specific radiosensitization.
    • The combination of DSF:Cu, gemcitabine, and ionizing radiation shows promise for cancer therapy.
    • Favorable toxicity profiles of DSF and gemcitabine support clinical evaluation.