Hydrogen peroxide is involved in hydrogen sulfide-induced lateral root formation in tomato seedlings

Both hydrogen sulfide (H₂S) and hydrogen peroxide (H₂O₂) are separately regarded as a highly reactive molecule involved in root morphogenesis. In this report, corresponding causal link governing lateral root formation was investigated.

By using pharmacological, anatomic, and molecular approaches, evidence presented here revealed the molecular mechanism underlying tomato lateral root development triggered by H₂S.

A H₂S donor sodium hydrosulfide (NaHS) triggered the accumulation of H₂O₂, the up-regulation of RBOH1 transcript, and thereafter tomato lateral root formation. Above responses were sensitive to the H₂O₂ scavenger (dimethylthiourea; DMTU) and the inhibitor of NADPH oxidase (diphenylene idonium; DPI), showing that the accumulations of H₂O₂ and increased RBOH1 transcript were respectively prevented. Lateral root primordial and lateral root formation were also impaired. Further molecular evidence revealed that H₂S-modulated gene expression of cell cycle regulatory genes, including up-regulation of SlCYCA2;1, SlCYCA3;1, and SlCDKA1, and the down-regulation of SlKRP2, were prevented by the co-treatment with DMTU or DPI. Above mentioned inducing phenotypes were consistent with the changes of lateral root formation-related microRNA transcripts: up-regulation of miR390a and miR160, and with the opposite tendencies of their target genes (encoding auxin response factors). Contrasting tendencies were observed when DMTU or DPI was added together. The occurrence of H₂S-mediated S-sulfhydration during above responses was preliminarily discovered.

Overall, these results suggested an important role of RBOH1-mediated H₂O₂ in H₂S-elicited tomato lateral root development, and corresponding H₂S-target proteins regulated at transcriptional and post-translational levels.