Ozone exposure effect on systemic prostaglandin F_2α in rat plasma and urine may not reveal pulmonary damage through...

The acute ozone induced lung injury model has been widely used to explore injury and repair processes induced by oxidant overload. The current study evaluated acute ozone exposure effects on prostaglandin F_2α (PGF_2α) in male Fischer rat plasma and urine with the hypothesis that ozone may induce an inflammatory response in the body that can be measured by the induction of PGF_2α. That might then lead to the identification of potential marker for acute lung injury through systemic inflammation. The time and dose-dependent effects of ozone exposure on the plasma and urinary levels of a major PGF_2α metabolite15-keto-dihydro-PGF_2α were determined using a radioimmunoassay. No statistically significant differences in the PGF_2α metabolite were found between the control and the experimental groups at either ozone exposure dose (2ppm and 5ppm) or any time point (2h, 7h and 16h) post exposure for plasma and at 7 different post exposure time points (between 2 and 80h) for urine. It is concluded that acute ozone exposure does not cause changes in plasma and urinary PGF_2α, and therefore their measurement in plasma and urine may not be used to reveal pulmonary inflammation and damage by ozone.