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Updated: Feb 20, 2026

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Optofluidic time-stretch quantitative phase microscopy.

Baoshan Guo1, Cheng Lei1, Yi Wu2

  • 1Department of Chemistry, University of Tokyo, Tokyo 113-0033, Japan.

Methods (San Diego, Calif.)
|October 17, 2017
PubMed
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This summary is machine-generated.

Optofluidic time-stretch quantitative phase microscopy enables high-throughput imaging of over 10,000 cells per second. This innovative technique overcomes speed limitations for advanced cell and particle characterization.

Area of Science:

  • Optics and Photonics
  • Biomedical Engineering
  • Microfluidics

Background:

  • Optical microscopy innovations have advanced scientific research, industrial quality control, and medical diagnostics.
  • Conventional quantitative phase imaging methods face speed limitations, hindering high-throughput analysis.

Purpose of the Study:

  • To review the principles of optofluidic time-stretch quantitative phase microscopy (OTSQPM).
  • To explore the diverse applications of OTSQPM in scientific and medical fields.
  • To discuss the future perspectives of this advanced imaging technique.

Main Methods:

  • Utilizes interference between temporally stretched signal and reference pulses.
  • Leverages dispersive properties of light in spatial and temporal domains.
Keywords:
High-throughput screeningMachine learningMicrofluidicsOptofluidic time-stretch microscopyQuantitative phase imagingSingle-cell analysis

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  • Employs an interferometric configuration on a microfluidic platform for continuous acquisition.

    • Main Results:

    • Achieves high-throughput quantitative phase imaging at over 10,000 particles or cells per second.
    • Overcomes speed limitations inherent in conventional quantitative phase imaging.
    • Enables continuous acquisition of both intensity and phase images.

    Conclusions:

    • Optofluidic time-stretch quantitative phase microscopy is a powerful tool for high-throughput cell and particle analysis.
    • Its applications span cancer cell characterization and microalgal culture analysis.
    • The technology holds significant promise for future advancements in various scientific disciplines.