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Related Experiment Videos

Modulating signaling networks by CRISPR/Cas9-mediated transposable element insertion.

Luis María Vaschetto1,2

  • 1Instituto de Diversidad y Ecología Animal, Consejo Nacional de Investigaciones Científicas y Técnicas (IDEA, CONICET), Av. Vélez Sarsfield 299, X5000JJC, Córdoba, Argentina. luisvaschetto@hotmail.com.

Current Genetics
|October 17, 2017
PubMed
Summary

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Transposable elements (TEs) are now understood to drive evolution and adaptive fitness. Miniature inverted-repeat transposable elements (MITEs), combined with CRISPR/Cas9 technology, offer new ways to control host gene expression.

Area of Science:

  • Genetics
  • Molecular Biology
  • Evolutionary Biology

Background:

  • Transposable elements (TEs) were historically viewed as selfish genetic entities.
  • TEs are now recognized as crucial evolutionary mechanisms enhancing adaptive fitness.
  • The manipulation of TEs for host function control was previously limited by genome engineering tools.

Purpose of the Study:

  • To explore the potential of transposable elements (TEs) for controlling host transcriptional activity.
  • To highlight miniature inverted-repeat transposable elements (MITEs) as promising tools for functional driver design.
  • To review recent advancements in MITEs and CRISPR/Cas9 technology for TE manipulation.

Main Methods:

  • Review of current literature on transposable elements and genome engineering.
Keywords:
CRISPR/Cas9Genome editingMiniature inverted transposable elementsTE-based drivesTranscriptional controlTransposable element amplification

Related Experiment Videos

  • Focus on miniature inverted-repeat transposable elements (MITEs) and their characteristics.
  • Integration of RNA-guided CRISPR/Cas9 endonuclease systems for TE deployment.

    • Main Results:

    • TEs play a significant role in genome evolution and adaptive fitness.
    • Miniature inverted-repeat transposable elements (MITEs) possess unique features suitable for developing functional drivers.
    • CRISPR/Cas9 technology enables the manipulation of TEs for controlling host gene expression.

    Conclusions:

    • The understanding of TEs has evolved from selfish elements to drivers of evolution.
    • MITEs present a viable strategy for engineering host transcriptional control.
    • The synergy between MITEs and CRISPR/Cas9 opens new avenues for functional genomics and biotechnology.