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Borrelia burgdorferi Transcriptome Analysis by RNA-Sequencing.

M Lybecker1, K C Henderson2

  • 1Department of Biology, University of Colorado Colorado Springs, 1420 Austin Bluffs Pkwy, Colorado Springs, CO, 80918, USA. meghan.lybecker@uccs.edu.

Methods in Molecular Biology (Clifton, N.J.)
|October 17, 2017
PubMed
Summary

Next-Generation Sequencing (NGS) provides advanced transcriptome analysis, enabling the discovery of novel transcripts and accurate gene expression profiling. This study details a new method for creating RNA sequencing libraries in Borrelia burgdorferi.

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Area of Science:

  • Molecular Biology
  • Genomics
  • Transcriptomics

Background:

  • Next-Generation Sequencing (NGS) has become a powerful tool in transcriptomics research.
  • NGS-based RNA sequencing offers advantages over DNA microarrays, including the identification of novel transcripts and transcriptional start sites.
  • RNA sequencing allows for simultaneous differential gene expression analysis of both known and unannotated transcripts.

Purpose of the Study:

  • To describe an Illumina-compatible, ligation-based protocol for generating stranded cDNA libraries.
  • To enable comprehensive transcriptome analysis in Borrelia burgdorferi.
  • To facilitate the study of both total RNA and small RNA populations.

Main Methods:

  • Development of a ligation-based cDNA library preparation method.
Keywords:
Next-generation sequencingRNA-sequencingTranscriptomics

Related Experiment Videos

  • Adaptation for Illumina sequencing platforms.
  • Application to total RNA and small RNA from Borrelia burgdorferi.

    • Main Results:

    • A robust protocol for generating stranded cDNA libraries for RNA sequencing.
    • Successful application to Borrelia burgdorferi transcriptomes.
    • Enables detailed analysis of both large and small RNA species.

    Conclusions:

    • The described method provides a reliable approach for stranded RNA library preparation.
    • Facilitates advanced transcriptomic studies in Borrelia burgdorferi.
    • Advances the understanding of gene expression in this bacterium.