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Related Experiment Videos

Microfluidic Iterative Mechanical Characteristics (iMECH) Analyzer for Single-Cell Metastatic Identification.

Hesam Babahosseini1,2, Jeannine S Strobl2, Masoud Agah2

  • 1Department of Mechanical Engineering, Virginia Tech, Blacksburg, VA 24061, US.

Analytical Methods : Advancing Methods and Applications
|October 17, 2017
PubMed
Summary

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Analytical chemistry·2021

A new microfluidic biosensor, the iterative mechanical characteristics (iMECH) analyzer, distinguishes metastatic from non-metastatic breast cancer cells. It uses label-free biomechanical profiling to detect distinct cellular responses to cyclic deformation, aiding cancer diagnostics.

Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Cancer Research

Background:

  • Metastatic cancer cells exhibit altered biomechanical properties compared to non-metastatic cells.
  • Pulsed mechanical nanoindentation can differentiate cell types based on biomechanical responses.
  • A microfluidic platform offers a clinically relevant approach for cell analysis.

Purpose of the Study:

  • To develop a microfluidic biosensor, the iterative mechanical characteristics (iMECH) analyzer, for label-free biomechanical profiling of individual cells.
  • To distinguish between metastatic and non-metastatic human mammary cell lines using the iMECH analyzer.
  • To establish a novel diagnostic tool for single-cell level metastatic cancer discrimination.

Main Methods:

  • Development of the iterative mechanical characteristics (iMECH) analyzer, a microfluidic device.
Keywords:
breast cancercell mechanicsmicrofluidics chipsingle-cell analysis

Related Experiment Videos

  • Application of cyclic deformation by pulling cells through narrow test channels and wider relaxation regions.
  • Label-free biomechanical profiling of human breast cell lines (184A1, MCF10A, MDA-MB-231, MDA-MB-468).
  • Analysis of cell velocity changes in response to cyclic deformation within the microfluidic channels.

    • Main Results:

    • Non-metastatic cells (184A1, MCF10A) showed increased resistance, indicated by a velocity drop after relaxation.
    • Metastatic cells (MDA-MB-231, MDA-MB-468) exhibited reduced resistance, shown by a velocity increase after relaxation.
    • Distinct biomechanical responses to cyclic deformation created a unique bio-signature for cell types.

    Conclusions:

    • The iMECH analyzer successfully differentiates metastatic from non-metastatic breast cancer cells based on their biomechanical profiles.
    • The observed differential mechanical responses provide a novel method for cancer cell characterization.
    • The iMECH analyzer represents a significant advancement in microchip-based diagnostics for single-cell metastatic cancer detection.