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Related Experiment Video

Updated: Jul 16, 2026

Generation and Culture of Blood Outgrowth Endothelial Cells from Human Peripheral Blood

Generation and Culture of Blood Outgrowth Endothelial Cells from Human Peripheral Blood

Published on: December 23, 2015

Young endothelial cells revive aging blood.

Vivian Y Chang¹, Christina M Termini², John P Chute^2,3,4

¹Division of Hematology/Oncology, Department of Pediatrics.

The Journal of Clinical Investigation

|October 17, 2017

Summary

Infusing young bone marrow endothelial cells (BMECs) rejuvenates the aging hematopoietic system in mice. This approach restores immune cell numbers and improves hematopoietic stem cell function, offering potential therapeutic strategies.

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Murine Dermal Lymphatic Endothelial Cell Isolation

Murine Dermal Lymphatic Endothelial Cell Isolation

Published on: July 21, 2023

Area of Science:

Hematology
Immunology
Aging Research

Background:

Aging leads to hematopoietic system decline, characterized by reduced hematopoietic stem cell (HSC) self-renewal, myeloid skewing, and immune cell depletion.
These age-related changes increase the risk of myeloid malignancies and impair adaptive immunity, highlighting the need for rejuvenation strategies.

Purpose of the Study:

To investigate the potential of bone marrow endothelial cells (BMECs) from young mice to rejuvenate the aging hematopoietic system.
To assess the impact of BMEC infusions on HSC self-renewal, immune cell restoration, and HSC engraftment in aged mice.

Main Methods:

Infusion of bone marrow endothelial cells (BMECs) from young mice into aged mice.
Co-delivery of young BMECs and HSCs following total body irradiation in aged mice.
Evaluation of HSC self-renewal, immune cell content, HSC engraftment, and survival rates.

Main Results:

Infusions of young BMECs promoted HSC self-renewal and restored immune cell content in aged mice.
Delivery of young BMECs alongside HSCs improved HSC engraftment and enhanced survival post-irradiation.
These findings indicate a crucial role for BMECs in mitigating hematopoietic aging.

Conclusions:

Bone marrow endothelial cells (BMECs) play a significant role in regulating hematopoietic aging.
Young BMECs possess rejuvenating properties that can restore HSC function and immune repertoire in aged individuals.
Further research is warranted to identify specific rejuvenating factors from BMECs for therapeutic development.