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Linear Chain Formation of Split-Aptamer Dimers on Surfaces Triggered by Adenosine
Chenze Lu1,2, Christine Saint-Pierre1, Didier Gasparutto1
1Univ. Grenoble Alpes, CEA, CNRS, INAC, SyMMES , F-38000 Grenoble, France.
Langmuir : the ACS Journal of Surfaces and Colloids
|October 17, 2017
Summary
This study introduces a novel biosensor for detecting small molecules like adenosine. The biosensor utilizes split aptamers and surface plasmon resonance imaging for enhanced signal amplification and detection.
Area of Science:
- Biotechnology
- Analytical Chemistry
- Molecular Biology
Background:
- Detection of small molecules is crucial but challenging due to their size and low concentrations.
- Existing biosensors require effective probes and signal amplification strategies.
- Aptamers offer specific molecular recognition for targets like adenosine.
Purpose of the Study:
- To develop an original amplification approach for small molecule detection using split aptamers.
- To investigate the performance of this approach with adenosine as a target.
- To analyze the impact of probe grafting density on chain formation and biosensor performance.
Main Methods:
- Utilized split aptamer sequences for sandwich assays.
- Combined self-assembling oligonucleotide dimers with split-aptamer dangling ends.
- Employed surface plasmon resonance imaging (SPRI) for signal detection.
- Performed sequence engineering and varied probe grafting density.
Main Results:
- Developed a novel amplification strategy based on linear chain formation triggered by adenosine.
- Demonstrated the biosensor's capability to detect adenosine.
- Analyzed the relationship between sequence engineering, grafting density, and chain length.
Conclusions:
- The developed biosensor offers a promising method for sensitive small molecule detection.
- Split aptamer-based linear chain formation is an effective signal amplification strategy.
- Optimizing probe density is key for enhancing biosensor performance and chain formation.