Related Experiment Video
Updated: Feb 20, 2026
Retroviral Scanning: Mapping MLV Integration Sites to Define Cell-specific Regulatory Regions
Published on: May 28, 2017
Ecotropic Viral Integration Site 5 (EVI5) expression analysis in multiple sclerosis patients
Mehrdokht Mazdeh1,2, Mahnoosh Rahimi3, Mohammad Mahdi Eftekharian1
1Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
Background:
Multiple sclerosis (MS) is a complex immune-related disorder of the central nervous system (CNS) in which dysregulation of different classes of T cells are involved. Variants in Ecotropic Viral Integration Site 5 (EVI5) gene has been shown to be significantly associated with MS in different populations.
Objectives:
However, there is no data regarding relative expression of this gene in peripheral blood of MS patients compared with healthy controls.
Methods:
In the present study we assessed expression of EVI5 in 50 Iranian MS patients compared with healthy subjects by means of quantitative real time RT-PCR.
Results:
Statistical analyses showed no significant difference in EVI5 relative expression neither between total MS patients and healthy controls nor between age- and sex-based subgroups of patients and controls except for a trend toward significance in patients aged between 30 and 40 years compared with healthy subjects in both sexes (P= 0.068 and 0.075 for males and females respectively). No significant correlation was found between the expression level of this gene and disease duration, age at onset or Expanded Disability Status Scale (EDSS).
Conclusion:
Future studies are needed to explore the role of EVI5 in the pathogenesis of MS.
Insights
This study found no significant difference in Ecotropic Viral Integration Site 5 (EVI5) gene expression in the blood of multiple sclerosis (MS) patients compared to healthy individuals. Further research is needed to understand EVI5's role in MS.
Area of Science:
- Neuroimmunology
- Genetics
- Molecular Biology
Background:
- Multiple sclerosis (MS) is an immune-mediated central nervous system disorder involving T cell dysregulation.
- Genetic variants in the Ecotropic Viral Integration Site 5 (EVI5) gene are associated with MS susceptibility.
- Limited data exists on EVI5 gene expression levels in MS patients.
Purpose of the Study:
- To investigate the relative expression of the EVI5 gene in peripheral blood of MS patients versus healthy controls.
- To determine if EVI5 expression correlates with clinical parameters in MS.
Main Methods:
- Quantitative real-time RT-PCR was used to measure EVI5 gene expression.
- The study included 50 Iranian MS patients and healthy controls.
- Expression levels were compared between groups and correlated with disease duration, age at onset, and EDSS.
Main Results:
- No statistically significant difference in EVI5 relative expression was observed between MS patients and healthy controls.
- A trend towards significance was noted in patients aged 30-40 years.
- EVI5 expression did not correlate with disease duration, age at onset, or EDSS.
Conclusions:
- The study did not find a significant difference in EVI5 expression in MS patients.
- Further investigation is required to elucidate the role of EVI5 in the pathogenesis of multiple sclerosis.
