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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: Feb 20, 2026

Identification of a Murine Erythroblast Subpopulation Enriched in Enucleating Events by Multi-spectral Imaging Flow Cytometry

Identification of a Murine Erythroblast Subpopulation Enriched in Enucleating Events by Multi-spectral Imaging Flow Cytometry

Published on: June 6, 2014

Eosinophilic otitis media diagnosis using flow cytometric immunophenotyping.

Issam Saliba¹, Musaed Alzahrani¹, Xiaoduan Weng²

¹a Division of Otolaryngology - Head and Neck Surgery , Montreal University Hospital Center (CHUM), University of Montreal , Montreal , QC , Canada.

Acta Oto-Laryngologica

|October 18, 2017

Summary

Flow cytometry can help diagnose eosinophilic otitis media (EOM) by detecting eosinophils in middle ear effusion. While effective, treatment responses were temporary, and EOM was not consistently linked to bronchial asthma.

Keywords:

Eosinophilic otitis media flow cytometry immunophenotyping middle ear effusion

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Last Updated: Feb 20, 2026

Identification of a Murine Erythroblast Subpopulation Enriched in Enucleating Events by Multi-spectral Imaging Flow Cytometry

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Published on: June 6, 2014

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Published on: November 16, 2021

Identification and Characterization of Immunogenic RNA Species in HDM Allergens that Modulate Eosinophilic Lung Inflammation

Identification and Characterization of Immunogenic RNA Species in HDM Allergens that Modulate Eosinophilic Lung Inflammation

Published on: May 30, 2020

Area of Science:

Otolaryngology
Allergy and Immunology
Medical Diagnostics

Background:

Eosinophilic otitis media (EOM) is a distinct inflammatory condition of the middle ear.
The role of eosinophils in EOM pathogenesis requires precise diagnostic methods.
The association between EOM and bronchial asthma remains an area of investigation.

Purpose of the Study:

To evaluate flow cytometric immunophenotyping for detecting and quantifying eosinophils in EOM.
To assess the relationship between EOM and bronchial asthma.
To monitor treatment response using flow cytometry.

Main Methods:

Prospective cohort study of 21 patients with chronic otorrhea or middle ear effusion (MEE).
MEE samples analyzed via flow cytometry for eosinophil counts.
Patients with positive eosinophils treated with dexamethasone eardrops and re-evaluated.

Main Results:

EOM diagnosed in all asthma-associated patients and 3 non-asthma patients.
Significantly higher mean eosinophil percentages in asthma-associated EOM (43.5%) vs. non-asthma EOM (14.2%).
Flow cytometry showed significant eosinophil reduction post-dexamethasone, but symptoms recurred; asthma association was not universal.

Conclusions:

Flow cytometry is a potentially valuable tool for diagnosing and monitoring EOM, especially in non-asthmatic patients.
Clinical diagnosis remains primary, with flow cytometry serving as an adjunct.
Temporary response to dexamethasone highlights the chronic nature of EOM.