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Electroporation-induced transformation of intact cells of Clostridium perfringens
1Department of Food Science, University of Illinois, Urbana 61801.
Applied and Environmental Microbiology
|September 1, 1988
Abstract:
Electroporation-induced transformation of intact cells of Clostridium perfringens 3624A with plasmids pAMB1 and pHR106 resulted in 3.8 X 10(-5) and 4.2 X 10(-4) transformants per viable cell, respectively. With respect to shuttle plasmid pHR106, these values represent a greater than 100-fold increase in transformation frequency when compared with the values reported with polyethylene glycol-induced L-phase variants.